Registry/Measurement kit/Notebook/2007-8-1: Difference between revisions
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*Started digest of promoters (E/S). | *Started digest of promoters (E/S). | ||
*Started another culture | *Started another culture | ||
*To do: ligate R0040 into the digest from the PCR and transform. | |||
==I2056== | ==I2056== | ||
*Purified digests (NotI, E/X, S/X) | *Purified digests (NotI, E/X, S/X) | ||
*Re-sealed and transformed. | |||
*To do: start choosing some RBSs to test | |||
==I2055== | ==I2055== | ||
*Running gel of BioBrick PCR. | *Running gel of BioBrick PCR. | ||
[[Image:8-1.jpg|thumb|left]] | [[Image:8-1.jpg|thumb|left]] | ||
*(ladder, I2055-EX, I2055-SX) | |||
*Both look right size. | *Both look right size. | ||
*Restriction digests: E/X, S/X, and NotI. | *Restriction digests: E/X, S/X, and NotI. | ||
==T9002== | |||
*Re-did scarring PCR of F2620. | |||
Revision as of 11:53, 1 August 2007
I2057
- Started digest of promoters (E/S).
- Started another culture
- To do: ligate R0040 into the digest from the PCR and transform.
I2056
- Purified digests (NotI, E/X, S/X)
- Re-sealed and transformed.
- To do: start choosing some RBSs to test
I2055
- Running gel of BioBrick PCR.
- (ladder, I2055-EX, I2055-SX)
- Both look right size.
- Restriction digests: E/X, S/X, and NotI.
T9002
- Re-did scarring PCR of F2620.
