Registry/Measurement kit/Notebook/2007-8-1: Difference between revisions
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*Started digest of promoters (E/S). | *Started digest of promoters (E/S). | ||
*Started another culture | *Started another culture | ||
*To do: ligate R0040 into the | *To do: ligate R0040 into the digests from the PCR and transform. | ||
==I2056== | ==I2056== |
Revision as of 12:00, 1 August 2007
I2057
- Started digest of promoters (E/S).
- Started another culture
- To do: ligate R0040 into the digests from the PCR and transform.
I2056
- Purified digests (NotI, E/X, S/X)
- Re-sealed and transformed.
- To do: start choosing some RBSs to test
I2055
- Running gel of BioBrick PCR.
- (ladder, I2055-EX, I2055-SX)
- Both look right size.
- Restriction digests: E/X, S/X, and NotI.
T9002
- Re-did scarring PCR of F2620.