Registry/Measurement kit/Notebook/2007-8-3: Difference between revisions
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*Should make more MG1655. | *Should make more MG1655. | ||
*Re-tried yesterday's transformations into Top10s. | *Re-tried yesterday's transformations into Top10s. | ||
*O/n of MG1655 is growing. | |||
Latest revision as of 13:51, 3 August 2007
E0240
- Trying mutagenic PCR of E0240.
- Used 17 cycles. Don't know what # is optimal. Stratagene recommends 12, but people seem to use ~18.
- Too many cycles -> more junk DNA.
- Digesting with DpnI. Felix suggested digesting for >2h.
Transformations
- Later will try re-transforming into another set of competent Top10 cells.
- Should make more MG1655.
- Re-tried yesterday's transformations into Top10s.
- O/n of MG1655 is growing.
