Registry/Measurement kit/Notebook/2007-8-9: Difference between revisions
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(New page: *Reviewed sequencing results: **None of E0240 worked except E0240-EX, E2. Not sure about ES, M2. **F2620 isn't right, should try re-ligating and transformibg in DB3.1 **55-SX-B0031 was ba...) |
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*Reviewed sequencing results: | *Reviewed sequencing results: | ||
**None of E0240 worked except E0240-EX, E2. Not sure about ES, M2. | **None of E0240 worked except E0240-EX, E2. Not sure about ES, M2. | ||
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**57 EX "J116" (actually 102): #1 has mutated beginning, #2 could be a poor read | **57 EX "J116" (actually 102): #1 has mutated beginning, #2 could be a poor read | ||
**57 EX R0040: both (1&2) are good. | **57 EX R0040: both (1&2) are good. | ||
*Should try more colonies of E0240 | |||
*making o/n of B0034 (S/X) I2055. two colonies worked. | |||
**perhaps the S/X construct is too difficult to use? | |||
Latest revision as of 13:51, 9 August 2007
- Reviewed sequencing results:
- None of E0240 worked except E0240-EX, E2. Not sure about ES, M2.
- F2620 isn't right, should try re-ligating and transformibg in DB3.1
- 55-SX-B0031 was backwards
- 57 ES r0040 #1 is good.
- 57 EX "J116" (actually 102): #1 has mutated beginning, #2 could be a poor read
- 57 EX R0040: both (1&2) are good.
- Should try more colonies of E0240
- making o/n of B0034 (S/X) I2055. two colonies worked.
- perhaps the S/X construct is too difficult to use?
