Registry/Measurement kit/Notebook/2007-8-9

From OpenWetWare

< Registry | Measurement kit | Notebook(Difference between revisions)
Jump to: navigation, search
(New page: *Reviewed sequencing results: **None of E0240 worked except E0240-EX, E2. Not sure about ES, M2. **F2620 isn't right, should try re-ligating and transformibg in DB3.1 **55-SX-B0031 was ba...)
Current revision (15:51, 9 August 2007) (view source)
 
Line 1: Line 1:
-
 
*Reviewed sequencing results:
*Reviewed sequencing results:
**None of E0240 worked except E0240-EX, E2. Not sure about ES, M2.
**None of E0240 worked except E0240-EX, E2. Not sure about ES, M2.
Line 7: Line 6:
**57 EX "J116" (actually 102): #1 has mutated beginning, #2 could be a poor read
**57 EX "J116" (actually 102): #1 has mutated beginning, #2 could be a poor read
**57 EX R0040: both (1&2) are good.
**57 EX R0040: both (1&2) are good.
 +
*Should try more colonies of E0240
 +
 +
*making o/n of B0034 (S/X) I2055. two colonies worked.
 +
**perhaps the S/X construct is too difficult to use?

Current revision

  • Reviewed sequencing results:
    • None of E0240 worked except E0240-EX, E2. Not sure about ES, M2.
    • F2620 isn't right, should try re-ligating and transformibg in DB3.1
    • 55-SX-B0031 was backwards
    • 57 ES r0040 #1 is good.
    • 57 EX "J116" (actually 102): #1 has mutated beginning, #2 could be a poor read
    • 57 EX R0040: both (1&2) are good.
  • Should try more colonies of E0240
  • making o/n of B0034 (S/X) I2055. two colonies worked.
    • perhaps the S/X construct is too difficult to use?
Personal tools