Registry/Measurement kit/Notebook/Design: Difference between revisions
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*Which one? | *Which one? | ||
*previous work (<bbpart>BBa_B0015</bbpart>) | *previous work (<bbpart>BBa_B0015</bbpart>) | ||
==Proposed kit designs== | |||
===BioBrick tester with GFP=== | |||
*E00240 | |||
===BioBrick tester with RFP=== | |||
*B0032 + (J04650 or I13501) | |||
*I13503 + B0015 | |||
===RBS tester with GFP=== | |||
*R0040 + (I13401 or J04630) | |||
===RBS tester with RFP=== | |||
R0040 + (J04650 or I13501) | |||
Revision as of 11:46, 11 June 2007
Design considerations for the expression measurement kit
Promoter
- Constituitive?
- Relative strength
- Location of transcription initiation (outside of or within the promoter?)
- previous work
Ribosome Binding Site
- Relative Strength
- Previous work (<bbpart>BBa_B0032</bbpart>)
Fluorescent Protein
- Color?
- previous work (<bbpart>E0040</bbpart>)
- Tagged?
Terminator
- Do we need one?
- Which one?
- previous work (<bbpart>BBa_B0015</bbpart>)
Proposed kit designs
BioBrick tester with GFP
- E00240
BioBrick tester with RFP
- B0032 + (J04650 or I13501)
- I13503 + B0015
RBS tester with GFP
- R0040 + (I13401 or J04630)
RBS tester with RFP
R0040 + (J04650 or I13501)
