Registry/Measurement kit/Preparation: Difference between revisions
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*<bbpart>E0240</bbpart> | *<bbpart>E0240</bbpart> | ||
*TOP10 Chemically competent cells | *TOP10 Chemically competent cells | ||
==Preparation== | |||
===psb3K3=== | |||
*The psb3K3 "backbone" plasmid is produced by PCR from a prepped psb3K3 template. | |||
*A preparative PCR mix containing: | |||
**190 of PCR Supermix HIFI from Invitrogen | |||
**5uL of 3K3SuffixFWD primer | |||
**5uL of 3K3PrefixREV primer | |||
**0.5uL of template at ~40ng/uL | |||
*Run PCR with the following conditions: | |||
*#95C for 5 min | |||
*#95C for 30 sec | |||
*#55C for 30 sec | |||
*#68C for 3min 20sec | |||
*#Goto 2 35 times | |||
*#68C for 10 min | |||
*#4C for eva | |||
=RBS Measurement Kit= | =RBS Measurement Kit= | ||
Revision as of 08:27, 14 January 2008
Description of the measurement kit components and methods of preparation.
Promoter Measurement Kit
Components
- psb3K3
- <bbpart>E0240</bbpart>
- TOP10 Chemically competent cells
Preparation
psb3K3
- The psb3K3 "backbone" plasmid is produced by PCR from a prepped psb3K3 template.
- A preparative PCR mix containing:
- 190 of PCR Supermix HIFI from Invitrogen
- 5uL of 3K3SuffixFWD primer
- 5uL of 3K3PrefixREV primer
- 0.5uL of template at ~40ng/uL
- Run PCR with the following conditions:
- 95C for 5 min
- 95C for 30 sec
- 55C for 30 sec
- 68C for 3min 20sec
- Goto 2 35 times
- 68C for 10 min
- 4C for eva
RBS Measurement Kit
Components
- psb3K3
- <bbpart>I13401</bbpart>
- TOP10 Chemically competent cells
