Registry of Standard Biological Models/Basic Component Models/Repressed Promoter RBS Coupled: Difference between revisions
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*'''Component''': RepressedPromoterRBSCoupled | *'''Component''': RepressedPromoterRBSCoupled | ||
*'''Units''': | *'''Units''': | ||
** | **Imported from Environment component | ||
*'''Variables''': | *'''Variables''': | ||
**nb-gene-copies (public interface = in / init value = 1.0) | **nb-gene-copies (public interface = in / init value = 1.0) | ||
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*'''MathML''' | *'''MathML''' | ||
**<amsmath>synthesisRate = \frac{nbGeneCopies*maxSynthesisRate}{K_d^{n}+[repressor]^{n}} </amsmath> | **<amsmath>synthesisRate = \frac{nbGeneCopies*maxSynthesisRate}{K_d^{n}+[repressor]^{n}} </amsmath> | ||
==Comments== | ==Comments== | ||
*This component is simply a container providing the synthesis rate specific to a constitutive promoter coupled to a RBS and a given level of the repressor | *This component is simply a container providing the synthesis rate specific to a constitutive promoter coupled to a RBS and a given level of the repressor | ||
*To characterize this part you need to get {maxSynthesisRate} and {sensitivity}. It can be achieved experimentally using a quantitative GFP assay. Measurement of GFP levels should be done at steady state with different levels of the repressor. | *To characterize this part you need to get {maxSynthesisRate} and {sensitivity}. It can be achieved experimentally using a quantitative GFP assay. Measurement of GFP levels should be done at steady state with different levels of the repressor. |
Revision as of 09:19, 9 September 2006
Repressed Promoter RBS Coupled Architecture
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CellML structure (CellML 1.1 spec)
- Component: RepressedPromoterRBSCoupled
- Units:
- Imported from Environment component
- Variables:
- nb-gene-copies (public interface = in / init value = 1.0)
- maxSynthesisRate (public interface = none / init value = XXX)
- Kd (dissociation constant) (public interface = none / init value = XXX)
- n (Hill Coefficient) (public interface = none / init value = XXX)
- synthesisRate (public interface = out / init value = XXX)
- repressor (public interface = in )
- MathML
- <amsmath>synthesisRate = \frac{nbGeneCopies*maxSynthesisRate}{K_d^{n}+[repressor]^{n}} </amsmath>
Comments
- This component is simply a container providing the synthesis rate specific to a constitutive promoter coupled to a RBS and a given level of the repressor
- To characterize this part you need to get {maxSynthesisRate} and {sensitivity}. It can be achieved experimentally using a quantitative GFP assay. Measurement of GFP levels should be done at steady state with different levels of the repressor.