Reiter:IPTG: Difference between revisions
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(New page: {{Template:Reiter}} == 1 M IPTG stock preparation== * 10 g IPTG * 41.960 mL sterile H<sub>2</sub>O # Vortex or shake until completely dissolved. # 0.22 μm filter into new 50 mL tube. # ...) |
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Latest revision as of 14:47, 5 March 2012
1 M IPTG stock preparation
- 10 g IPTG
- 41.960 mL sterile H2O
- Vortex or shake until completely dissolved.
- 0.22 μm filter into new 50 mL tube.
- Dispense 1 mL aliquots and freeze at -20 °C
