Research Idea Wiki Page

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(Background Information)
(Needed Resources)
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===Needed Resources===
===Needed Resources===
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Zinc Finger nucleases specific to CCR5.
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CCR5 delta 32 sequence and a construct with the CCR5delta32 sequence within arms of CCR5 sequences.
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KBM7 cells that are haploid for every chromosome except for chromosome 8. It will be easier to see the effects of a change in haploid cells because there won't be an ambiguous heterozygous phenotype.

Revision as of 03:42, 8 May 2013

Contents

Project Overview

We are looking to use zinc finger nucleases to modify the CCR5 receptor on genetically haploid cells to identify the role of CCR5 in HIV infection and to explore the possibility of generating immunity to HIV.

Background Information

CCR5 is a receptor on the outside of T cells that HIV uses to dalfdlsakjflas;kfjdlas;kfjs CCR5 delta 32 is an isoform of CCR5 found in certain Northern European individuals that confers resistance, or immunity, to HIV. Zinc Finger nucleases are tools that can be used to modify the human genome in a very target specific manner. Homology repair is a mechanism that cells use to repair damaged DNA. It is a mechanism that can be used to introduce new modified DNA sequences into the genome at very specific target locations.

Purpose and Goals

Project Details

Predicted Outcomes

Needed Resources

Zinc Finger nucleases specific to CCR5. CCR5 delta 32 sequence and a construct with the CCR5delta32 sequence within arms of CCR5 sequences. KBM7 cells that are haploid for every chromosome except for chromosome 8. It will be easier to see the effects of a change in haploid cells because there won't be an ambiguous heterozygous phenotype.

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