Restriction Digest 8-3: Difference between revisions
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The first 3 lanes are digeston from the first gel, I had some left over. The last 2 lanes are constructs 13 and 17 redone. Under the Treefort, I could see construct | The first 3 lanes are digeston from the first gel, I had some left over. The last 2 lanes are constructs 13 and 17 redone. Under the Treefort, I could see construct 17 very, very faintly. I continued to see nothing for 13. I then proceeded to do a colony PCR for 13 and got nothing. The gel is blank so it isn't interesting. | ||
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Revision as of 21:30, 3 August 2009
Below is a mapping of the 21 constructs. The constructs go from left to right in the below order:
# ID Construct Length
1 C09ig0027 {<VtaA11 AtD>}(dblterm) 378
2 C09ig0026 {<cl02365 AtD>}(dblterm) 963
3 C09ig0035 {<ehaB>}(dblterm) 1365
4 C09ig0028 {<Hag AtD>}(dblterm) 378
5 C09ig0024 {<azo1653 AtD>}(dblterm) 969
6 C09ig0029 {<Pcryo_1225 AtD>}(dblterm) 381
7 C09ig0025 {<OprF AtD>}(dblterm) 819
8 C09ig0030 {<Hia AtD>}(dblterm) 378
9 C09ig0031 {<upaG_short>}(dblterm) 405
10 C09ig0036 {<eCPX>}(dblterm) 612
11 C09ig0032 {<CPG_L2>}(dblterm) 999
12 C09ig0037 {<TshA>}(dblterm) 1710
13 C09ig0033 {<CPG_L6>}(dblterm) 999
14 C09ig0039 {<yuaQ AtD>}(dblterm) 1008
15 C09ig0034 {<espP(beta)>}(dblterm) 1488
16 C09ig0040 {<AIDA-1 AtD>}(dblterm) 1560
17 C09ig0019 {<Cub>}(dblterm) 273
18 C09ig0023 {<Tev C>}(dblterm) 513
19 C09ig0020 {<Nub>}(dblterm) 282
20 C09ig0021 {<caspace 3>}(dblterm) 969
21 C09ig0022 {<Tev N>}(dblterm) 495
The gel on the left is slightly bent which is why all of the constructs are ~100bp above what they should be. The two missing were 13 and 17. All of the constructs minus 13 and 17 could be seen under Dueber Treefort. A lot couldn't be seen from the blackbox.
There also seemed to be another faint band for 11, but the correct band was glowing brightly. I ran another gel on just 11, 13 and 17. on the second gel, from left to right, the ordering goes:
# ID Construct Length
11 C09ig0032 {<CPG_L2>}(dblterm) 999 From first digestion
13 C09ig0033 {<CPG_L6>}(dblterm) 999 From first digestion
17 C09ig0019 {<Cub>}(dblterm) 273 From first digestion
13 C09ig0033 {<CPG_L6>}(dblterm) 999 New digestion
17 C09ig0019 {<Cub>}(dblterm) 273 New digestion
The first 3 lanes are digeston from the first gel, I had some left over. The last 2 lanes are constructs 13 and 17 redone. Under the Treefort, I could see construct 17 very, very faintly. I continued to see nothing for 13. I then proceeded to do a colony PCR for 13 and got nothing. The gel is blank so it isn't interesting.
