Restriction Digest 8-3

Below is a mapping of the 21 constructs. The constructs go from left to right in the below order:

ID              Construct                           
C09ig0027	{<VtaA11 AtD>}(dblterm)	        378
C09ig0026	{<cl02365 AtD>}(dblterm)	963
C09ig0035	{<ehaB>}(dblterm)	        1365
C09ig0028	{<Hag AtD>}(dblterm)	        378
C09ig0024	{<azo1653 AtD>}(dblterm)	969
C09ig0029	{<Pcryo_1225 AtD>}(dblterm)	381
C09ig0025	{<OprF AtD>}(dblterm)	        819
C09ig0030	{<Hia AtD>}(dblterm)	        378
C09ig0031	{<upaG_short>}(dblterm)	        405
C09ig0036	{<eCPX>}(dblterm)	        612
C09ig0032	{<CPG_L2>}(dblterm)	        999
C09ig0037	{<TshA>}(dblterm)	        1710
C09ig0033	{<CPG_L6>}(dblterm)	        999
C09ig0039	{<yuaQ AtD>}(dblterm)	        1008
C09ig0034	{<espP(beta)>}(dblterm)	        1488
C09ig0040	{<AIDA-1 AtD>}(dblterm)         1560
C09ig0019	{<Cub>}(dblterm)	        273
C09ig0023	{<Tev C>}(dblterm)	        513
C09ig0020	{<Nub>}(dblterm)	        282
C09ig0021	{<caspace 3>}(dblterm)   	969
C09ig0022	{<Tev N>}(dblterm)	        495

The gel on the left is slightly bent which is why all of the constructs are ~100bp above what they should be.

I also ran a second gel to distinguish discrepancies between the constructs.