Restriction enzymes

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Current revision (09:26, 3 April 2008) (view source)
 
(6 intermediate revisions not shown.)
Line 2: Line 2:
Restriction enzymes are used for performing a [[restriction digest]].
Restriction enzymes are used for performing a [[restriction digest]].
-
Comprehensive information can be found in [http://rebase.neb.com/rebase/rebase.html REBASE].
+
Comprehensive information can be found in [http://rebase.neb.com/rebase/rebase.html REBASE]. [[NeerajCutterList|Neeraj Dhar's good cutter list]] is a list of enzymes which are particularly reliable and straightforwards.
 +
 
 +
This is another source of info on restricition enzymes. It's by the same people who provide REBASE, but in a different format:
 +
 
 +
 
 +
[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/buffer_activity_restriction_enzymes.asp Activity Chart for Restriction Enzymes]
 +
 
These wiki pages can contain people's experiences with using specific enzymes.
These wiki pages can contain people's experiences with using specific enzymes.
==Specific enzyme information==
==Specific enzyme information==
-
*[[DpnI]]
+
{{#dpl:category=Material|category=Enzyme|category=Endonuclease}}
-
*[[EcoRI]]
+
-
*[[PstI]]
+
-
*[[SpeI]]
+
-
*[[XbaI]]
+
-
*[[XmaI]]
+
-
 
+
-
===Offset Cutters===
+
[[Category:Material]] [[Category:Enzyme]] [[Category:Endonuclease]]
-
*[[BsmBI]]
+

Current revision

General information

Restriction enzymes are used for performing a restriction digest.

Comprehensive information can be found in REBASE. Neeraj Dhar's good cutter list is a list of enzymes which are particularly reliable and straightforwards.

This is another source of info on restricition enzymes. It's by the same people who provide REBASE, but in a different format:


Activity Chart for Restriction Enzymes


These wiki pages can contain people's experiences with using specific enzymes.

Specific enzyme information

Personal tools