Resuspension of primers
When receiving oligonucleotide primers from a manufacturer such as Invitrogen, the oligos arrive dry and must be resuspended in buffer.
Invitrogen recommends the following reconstitution procedure -
- Centrifuge the tube for a few seconds to get all the DNA to the bottom of the tube.
- Resuspend in TE buffer, pH 8.0 at a concentration greater than 10μM.
- Allow to sit for 2mins, then vortex for 15s.