Richard Lab:ADF: Difference between revisions
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| Line 5: | Line 5: | ||
==Materials== | ==Materials== | ||
*acid detergent solution (stored in room | *acid detergent solution (stored in room 113B) | ||
*2000 mL flask | *2000 mL flask | ||
*acetone (in flammables cabinet below fume hood in room 113B) | *acetone (in flammables cabinet below fume hood in room 113B) | ||
| Line 13: | Line 13: | ||
*digestion apparatus (ANKOM fiber analyzer) | *digestion apparatus (ANKOM fiber analyzer) | ||
*analytical balance | *analytical balance | ||
*drying oven at | *drying oven at 105°C | ||
*dessicator | *dessicator | ||
*zip-loc bags with dessicant pouches (recently recharged at | *zip-loc bags with dessicant pouches (recently recharged at 105°C) | ||
*hazardous waste containers | *hazardous waste containers | ||
Revision as of 13:20, 4 June 2009
Overview
Replace this sentence with a brief description of the protocol and its goal.
Materials
- acid detergent solution (stored in room 113B)
- 2000 mL flask
- acetone (in flammables cabinet below fume hood in room 113B)
- 4 glass jars
- microwave
- pH paper
- digestion apparatus (ANKOM fiber analyzer)
- analytical balance
- drying oven at 105°C
- dessicator
- zip-loc bags with dessicant pouches (recently recharged at 105°C)
- hazardous waste containers
Procedure
- Step 1
- Step 2
- Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
- Step 3
- Step 3 has multiple sub-steps within it.
- Enumerate each of those.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.
References
Relevant papers and books
- Goldbeter A and Koshland DE Jr. An amplified sensitivity arising from covalent modification in biological systems. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6840-4. DOI:10.1073/pnas.78.11.6840 |
- JACOB F and MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318-56. DOI:10.1016/s0022-2836(61)80072-7 |
- ISBN:0879697164
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.
