Richard Lab:ADL: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 14: | Line 14: | ||
*ANKOM Daisy incubator and jars | *ANKOM Daisy incubator and jars | ||
*analytical balance | *analytical balance | ||
*drying oven at | *drying oven at 105°C | ||
*dessicator | *dessicator | ||
*zip-loc bags with dessicant pouches (recently recharged at | *zip-loc bags with dessicant pouches (recently recharged at 105°C) | ||
*hazardous waste containers | *hazardous waste containers | ||
Revision as of 13:32, 4 June 2009
Overview
Replace this sentence with a brief description of the protocol and its goal.
Materials
- 2000 mL flask
- acetone (in flammables cabinet below fume hood in room 113B)
- 5 glass jars
- microwave
- pH paper
- concentrated sulfuric acid
- top-loading balance (1 decimal place)
- ANKOM Daisy incubator and jars
- analytical balance
- drying oven at 105°C
- dessicator
- zip-loc bags with dessicant pouches (recently recharged at 105°C)
- hazardous waste containers
Procedure
- Step 1
- Step 2
- Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
- Step 3
- Step 3 has multiple sub-steps within it.
- Enumerate each of those.
Notes
- ALWAYS add acid to water and work in fume hood when making 72% sulfuric acid
References
Relevant papers and books
- Goldbeter A and Koshland DE Jr. An amplified sensitivity arising from covalent modification in biological systems. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6840-4. DOI:10.1073/pnas.78.11.6840 |
- JACOB F and MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318-56. DOI:10.1016/s0022-2836(61)80072-7 |
- ISBN:0879697164
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.
