Richard Lab:ADL

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Contents

Overview

Replace this sentence with a brief description of the protocol and its goal.

Materials

  • 2000 mL flask
  • acetone (in flammables cabinet below fume hood in room 113B)
  • 5 glass jars
  • microwave
  • pH paper
  • concentrated sulfuric acid
  • top-loading balance (1 decimal place)
  • ANKOM Daisy incubator and jars
  • analytical balance
  • drying oven at 105°C
  • dessicator
  • zip-loc bags with dessicant pouches (recently recharged at 105°C)
  • hazardous waste containers

Procedure

  1. Step 1
  2. Step 2
    • Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
  3. Step 3
    1. Step 3 has multiple sub-steps within it.
    2. Enumerate each of those.

Notes

  • ALWAYS add acid to water and work in fume hood when making 72% sulfuric acid

References

Relevant papers and books

  1. Goldbeter A and Koshland DE Jr. . pmid:6947258. PubMed HubMed [Goldbeter-PNAS-1981]
  2. JACOB F and MONOD J. . pmid:13718526. PubMed HubMed [Jacob-JMB-1961]
  3. Mark Ptashne. A genetic switch. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press, 2004. isbn:0879697164. [Ptashne-Genetic-Switch]
All Medline abstracts: PubMed HubMed

Contact

  • Who has experience with this protocol?

or instead, discuss this protocol.


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