Richard Lab:ADL

Overview

Replace this sentence with a brief description of the protocol and its goal.

Materials

• 2000 mL flask
• acetone (in flammables cabinet below fume hood in room 113B)
• 5 glass jars
• microwave
• pH paper
• concentrated sulfuric acid
• top-loading balance (1 decimal place)
• ANKOM Daisy incubator and jars
• analytical balance
• drying oven at 105°C
• dessicator
• zip-loc bags with dessicant pouches (recently recharged at 105°C)
• hazardous waste containers

Procedure

1. Step 1
2. Step 2
   • Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
3. Step 3
   1. Step 3 has multiple sub-steps within it.
   2. Enumerate each of those.

Notes

• ALWAYS add acid to water and work in fume hood when making 72% sulfuric acid

References

Relevant papers and books

1. Goldbeter A and Koshland DE Jr. An amplified sensitivity arising from covalent modification in biological systems. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6840-4. DOI:10.1073/pnas.78.11.6840 |
2. JACOB F and MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318-56. DOI:10.1016/s0022-2836(61)80072-7 |
3. ISBN:0879697164

Contact

• Who has experience with this protocol?

or instead, discuss this protocol.