Richard Lab:ASE overview

Overview

• Automated extraction of solid samples with common solvents at elevated temperature and pressure (Fig. 1)
• Extraction steps: preheating sample cell (optional), filling cell with solvent, heating (equilibration), static extraction, rinsing, purging solvent and extractives into collection vessel, and relief
• Current applications
  • Water and 95% ethanol extractions for NREL biomass compositional analysis
  • Liquid hot water biomass pretreatment

Specifications

• Oven temperature
  • Ambient to 200oC
• Pressure
  • ~1600 psi
• Available sample cells (Fig. 2)
  • 13-10mL stainless steel (~1.5g dry biomass)
  • 6-66mL zirconium (~10g dry biomass)
  • 6-100mL zirconium
• Available extract collection vessels (Fig. 2)
  • 60mL vials
  • 250mL bottles

Sample Preparation Considerations

• Grinding
  • More surface area for efficient extraction
• Sample moisture
  • Samples may require mixing with drying (diatomaceous earth) or dispersing (Ottawa sand) agent
• Sample pH
  • Mildly acidic or basic samples can be extracted in zirconium cells

References

• Dionex info and application notes
  • http://www.dionex.com/en-us/products/sample-preparation/ase/lp-72855.html

Contact

• Who has experience with this protocol?

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