Richard Lab:Bio-Brick Formats: Difference between revisions
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==Introduction== | ==Introduction== | ||
In general the Richard Lab uses the standard [http://partsregistry.org/Help:BioBrick_Prefix_and_Suffix Bio-Brick] format. However occasionally this format is incompatible with our goals (e.g. making protein fusions) or is unnecessarilly cumbersome (e.g. PCR overlap extension). | In general the Richard Lab uses the standard [http://partsregistry.org/Help:BioBrick_Prefix_and_Suffix Bio-Brick] format. However occasionally this format is incompatible with our goals (e.g. making protein fusions) or is unnecessarilly cumbersome (e.g. PCR overlap extension). To have a review on why (and how) we use these sites see the our [[Richard Lab:Bio-Brick Assembly Schedule|New Standard Assembly]] page. | ||
==Formats== | ==Formats== | ||
Revision as of 13:27, 24 January 2011
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Introduction
In general the Richard Lab uses the standard Bio-Brick format. However occasionally this format is incompatible with our goals (e.g. making protein fusions) or is unnecessarilly cumbersome (e.g. PCR overlap extension). To have a review on why (and how) we use these sites see the our New Standard Assembly page.
Formats
Biobrick Standard
This standard uses the typical biobrick assembly enzymes [EcoRI (GAATTC), XbaI (TCTAGA), SpeI (ACTAGT), and PstI (CTGCAG)] as well as NotI sites (GCGGCCGC) flanking the part. There is a slightly different prefix for protein coding parts to put the start codon in a better spot in relation to the RBS.
- Prefix
- GAATTCgcggccgctTCTAGAg
- The EcoRI and XbaI sites are in CAPS while the NotI site is in bold.
- Protein Coding Prefix
- GAATTCgcggccgctTCTAG
- using this prefix the first base of the start codon (i.e. the "A" in "ATG") will complete the XbaI site.
- Suffix
- tACTAGTagcggccgCTGCAG
Short Biobrick
Protein Fusions
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