Richard Lab:IC eluents

System 1

Overview

IC System 1 consists of the ICEAS1 guard and analytical columns, a 750μL reaction coil, a UV detector (for organic acid detection), and an electrochemical detector with platinum electrode (for alcohol detection). The 4 eluent bottles are configured as listed here, with eluents A, B, and C requiring degassing: A = 100 mM methanesulfonic acid (2 L), B = 100 mM methanesulfonic acid (2 L), C = nanopure water (2 L), D = acetonitrile (1 L). Eluents A and B are used for running samples, while eluents C and D are available for system cleaning. A procedure for preparing eluents A and B is given below.

Materials

List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers' page on that material are also appropriate.

• supply 1 (i.e. tubes of a certain size? spreaders?)
• reagent 1
• X μL reagent 2
  • component A (reagent 2 is made up of multiple components)
  • component B
• equipment 1
• equipment 2

Procedure

1. Step 1
2. Step 2
   • Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
3. Step 3
   1. Step 3 has multiple sub-steps within it.
   2. Enumerate each of those.

Contact

• Who has experience with this protocol?

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System 2

Overview

Replace this sentence with a brief description of the protocol and its goal.

Materials

List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers' page on that material are also appropriate.

• supply 1 (i.e. tubes of a certain size? spreaders?)
• reagent 1
• X μL reagent 2
  • component A (reagent 2 is made up of multiple components)
  • component B
• equipment 1
• equipment 2

Procedure

1. Step 1
2. Step 2
   • Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
3. Step 3
   1. Step 3 has multiple sub-steps within it.
   2. Enumerate each of those.

Contact

• Who has experience with this protocol?

Back to Protocols