Richard Lab:Microorganisms & Media: Difference between revisions

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=Prokaryotic=
=Prokaryotic=
===''E. coli'' NEB 5a===
*A DH5-alpha derivitive supplied by [http://www.neb.com/nebecomm/products/productC2989.asp New England Biolabs]
*Used for general cloning including Bgal selection.
*Electrocompetent cells are kept in the -80°C Freezer in Dr. Demirci's lab.
*Ask [[user:Michael A. Speer|Mike]]
===''E. coli'' DB3.1===
*These clones are subject to ccdb lethality
*Plasmids with the ccdb gene surrounding the MCS are used to select for inserts
**DB3.1 cells with non insert plasmids will die
*Ask [[user:Michael A. Speer|Mike]]
===''Lactobacillus plantarum'' PA-28===
===''Lactobacillus plantarum'' PA-28===
*Commercially available silage inoculant
*Commercially available silage inoculant
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===''Saccharomyces cerevisiae'' NRRL Y-2034 (ATCC 24702)===
===''Saccharomyces cerevisiae'' NRRL Y-2034 (ATCC 24702)===
*Obtained from USDA-ARS Culture Collection
*Obtained from USDA-ARS Culture Collection
*Culture maintenance protocol
*Ask [[User:Megan_Marshall | Megan]]
Back to [[Richard Lab]]


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=Media=
===LB Lennox===
*
*
*
===SOB===
*
*
*
===SOC===
*
*
*
===MRS Medium===
*10g/L Peptone
*5g/L Yeast Extract
*5g/L Sodium Acetate
*2g/L Dipotassium Phosphate
*2g/l Ammonium Citrate
*1g/L Tween-80 (1ml polysorbate-80)
*0.1g/L Magnesium Sulfate
*0.05g/L Manganese Sulfate (137μL 38% Manganous Sulfate solution)
*Carbon source:
**For Full strength medium add 20g/L dextrose and 10g/L beef extract.
**For other purposes as little as 1.8g/L (10mM) dextrose can be used.
***If adjusting the pH with malate or arginine use 2.5g/L dextrose.
 
===M17===
*
*
*
===YPD===
*
*
*
===RMGX===

Latest revision as of 11:35, 20 October 2011

Back to Richard Lab Main Page

Prokaryotic

E. coli NEB 5a

  • A DH5-alpha derivitive supplied by New England Biolabs
  • Used for general cloning including Bgal selection.
  • Electrocompetent cells are kept in the -80°C Freezer in Dr. Demirci's lab.
  • Ask Mike

E. coli DB3.1

  • These clones are subject to ccdb lethality
  • Plasmids with the ccdb gene surrounding the MCS are used to select for inserts
    • DB3.1 cells with non insert plasmids will die
  • Ask Mike

Lactobacillus plantarum PA-28

  • Commercially available silage inoculant
  • Found in "Biomax 5" by CHR Hansen biosystems
  • Ask Mike

Lactobacillus plantarum K-270

  • Commercially available silage inoculant
  • Found in "Biomax 5" by CHR Hansen biosystems
  • Ask Mike

Lactobacillus plantarum LP-401

  • Commercially available silage inoculant
  • Found in "Biomax MP" by CHR Hansen biosystems
  • Ask Mike

Lactobacillus plantarum LP-1

  • Commercially available silage inoculant
  • Found in "Biomax MP" by CHR Hansen biosystems
  • Ask Mike

Lactobacillus plantarum MD007

  • is a knockout of the alanine racemase gene
  • cannot grow on MRS media without supplemented D-alanine
  • used for promoter probing with the pNZ7120 promoter probe vector
  • Bron, P.A., S. M. Hoffer, I. I. Van Swam, W. M. De Vos and M. Kleerebezem. 2003. Selection and Characterization of Conditionally Active Promoters in Lactobacillus plantarum, Using Alanine Racemase as a Promoter Probe. Applied and Environmental Microbiology 70 (1): 310–317
  • Ask Mike

Lactococcus lactis MG1363

  • Contains pNZ7120 promoter probe vector
  • Can be selected for based on ampicillin resistance
  • grow on M17 media supplemented with 0.5% glucose
  • Bron, P.A., S. M. Hoffer, I. I. Van Swam, W. M. De Vos and M. Kleerebezem. 2003. Selection and Characterization of Conditionally Active Promoters in Lactobacillus plantarum, Using Alanine Racemase as a Promoter Probe. Applied and Environmental Microbiology 70 (1): 310–317
  • Ask Mike

Pedicoccus pentosaceus PC-3

  • Commercially available silage inoculant
  • Found in "Biomax MP" by CHR Hansen biosystems
  • Ask Mike

Eukaryotic

Saccharomyces cerevisiae NRRL Y-2034 (ATCC 24702)

  • Obtained from USDA-ARS Culture Collection
  • Culture maintenance protocol
  • Ask Megan

Back to Richard Lab

Media

LB Lennox

SOB

SOC

MRS Medium

  • 10g/L Peptone
  • 5g/L Yeast Extract
  • 5g/L Sodium Acetate
  • 2g/L Dipotassium Phosphate
  • 2g/l Ammonium Citrate
  • 1g/L Tween-80 (1ml polysorbate-80)
  • 0.1g/L Magnesium Sulfate
  • 0.05g/L Manganese Sulfate (137μL 38% Manganous Sulfate solution)
  • Carbon source:
    • For Full strength medium add 20g/L dextrose and 10g/L beef extract.
    • For other purposes as little as 1.8g/L (10mM) dextrose can be used.
      • If adjusting the pH with malate or arginine use 2.5g/L dextrose.

M17

YPD

RMGX

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