Richard Lab:Microorganisms & Media

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(Saccharomyces cerevisiae NRRL Y-2034 (ATCC 24702))
(Saccharomyces cerevisiae NRRL Y-2034 (ATCC 24702))
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===''Saccharomyces cerevisiae'' NRRL Y-2034 (ATCC 24702)===
===''Saccharomyces cerevisiae'' NRRL Y-2034 (ATCC 24702)===
*Obtained from USDA-ARS Culture Collection
*Obtained from USDA-ARS Culture Collection
 +
*Culture maintenance protocol
*Ask [[User:Megan_Marshall | Megan]]
*Ask [[User:Megan_Marshall | Megan]]
Back to [[Richard Lab]]
Back to [[Richard Lab]]

Revision as of 19:19, 9 February 2010

Back to Richard Lab

Contents

Prokaryotic

E. coli NEB 5a

  • A DH5-alpha derivitive supplied by New England Biolabs
  • Used for general cloning including Bgal selection.
  • Electrocompetent cells are kept in the -80°C Freezer in Dr. Demirci's lab.
  • Ask Mike

E. coli DB3.1

  • These clones are subject to ccdb lethality
  • Plasmids with the ccdb gene surrounding the MCS are used to select for inserts
    • DB3.1 cells with non insert plasmids will die
  • Ask Mike

Lactobacillus plantarum PA-28

  • Commercially available silage inoculant
  • Found in "Biomax 5" by CHR Hansen biosystems
  • Ask Mike

Lactobacillus plantarum K-270

  • Commercially available silage inoculant
  • Found in "Biomax 5" by CHR Hansen biosystems
  • Ask Mike

Lactobacillus plantarum LP-401

  • Commercially available silage inoculant
  • Found in "Biomax MP" by CHR Hansen biosystems
  • Ask Mike

Lactobacillus plantarum LP-1

  • Commercially available silage inoculant
  • Found in "Biomax MP" by CHR Hansen biosystems
  • Ask Mike

Lactobacillus plantarum MD007

  • is a knockout of the alanine racemase gene
  • cannot grow on MRS media without supplemented D-alanine
  • used for promoter probing with the pNZ7120 promoter probe vector
  • Bron, P.A., S. M. Hoffer, I. I. Van Swam, W. M. De Vos and M. Kleerebezem. 2003. Selection and Characterization of Conditionally Active Promoters in Lactobacillus plantarum, Using Alanine Racemase as a Promoter Probe. Applied and Environmental Microbiology 70 (1): 310–317
  • Ask Mike

Lactococcus lactis MG1363

  • Contains pNZ7120 promoter probe vector
  • Can be selected for based on ampicillin resistance
  • grow on M17 media supplemented with 0.5% glucose
  • Bron, P.A., S. M. Hoffer, I. I. Van Swam, W. M. De Vos and M. Kleerebezem. 2003. Selection and Characterization of Conditionally Active Promoters in Lactobacillus plantarum, Using Alanine Racemase as a Promoter Probe. Applied and Environmental Microbiology 70 (1): 310–317
  • Ask Mike

Pedicoccus pentosaceus PC-3

  • Commercially available silage inoculant
  • Found in "Biomax MP" by CHR Hansen biosystems
  • Ask Mike

Eukaryotic

Saccharomyces cerevisiae NRRL Y-2034 (ATCC 24702)

  • Obtained from USDA-ARS Culture Collection
  • Culture maintenance protocol
  • Ask Megan

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