Richard Lab:Microorganisms & Media

From OpenWetWare

Revision as of 13:35, 20 October 2011 by Michael A. Speer (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search

Back to Richard Lab Main Page

Contents

Prokaryotic

E. coli NEB 5a

  • A DH5-alpha derivitive supplied by New England Biolabs
  • Used for general cloning including Bgal selection.
  • Electrocompetent cells are kept in the -80°C Freezer in Dr. Demirci's lab.
  • Ask Mike

E. coli DB3.1

  • These clones are subject to ccdb lethality
  • Plasmids with the ccdb gene surrounding the MCS are used to select for inserts
    • DB3.1 cells with non insert plasmids will die
  • Ask Mike

Lactobacillus plantarum PA-28

  • Commercially available silage inoculant
  • Found in "Biomax 5" by CHR Hansen biosystems
  • Ask Mike

Lactobacillus plantarum K-270

  • Commercially available silage inoculant
  • Found in "Biomax 5" by CHR Hansen biosystems
  • Ask Mike

Lactobacillus plantarum LP-401

  • Commercially available silage inoculant
  • Found in "Biomax MP" by CHR Hansen biosystems
  • Ask Mike

Lactobacillus plantarum LP-1

  • Commercially available silage inoculant
  • Found in "Biomax MP" by CHR Hansen biosystems
  • Ask Mike

Lactobacillus plantarum MD007

  • is a knockout of the alanine racemase gene
  • cannot grow on MRS media without supplemented D-alanine
  • used for promoter probing with the pNZ7120 promoter probe vector
  • Bron, P.A., S. M. Hoffer, I. I. Van Swam, W. M. De Vos and M. Kleerebezem. 2003. Selection and Characterization of Conditionally Active Promoters in Lactobacillus plantarum, Using Alanine Racemase as a Promoter Probe. Applied and Environmental Microbiology 70 (1): 310–317
  • Ask Mike

Lactococcus lactis MG1363

  • Contains pNZ7120 promoter probe vector
  • Can be selected for based on ampicillin resistance
  • grow on M17 media supplemented with 0.5% glucose
  • Bron, P.A., S. M. Hoffer, I. I. Van Swam, W. M. De Vos and M. Kleerebezem. 2003. Selection and Characterization of Conditionally Active Promoters in Lactobacillus plantarum, Using Alanine Racemase as a Promoter Probe. Applied and Environmental Microbiology 70 (1): 310–317
  • Ask Mike

Pedicoccus pentosaceus PC-3

  • Commercially available silage inoculant
  • Found in "Biomax MP" by CHR Hansen biosystems
  • Ask Mike

Eukaryotic

Saccharomyces cerevisiae NRRL Y-2034 (ATCC 24702)

  • Obtained from USDA-ARS Culture Collection
  • Culture maintenance protocol
  • Ask Megan

Back to Richard Lab

Media

LB Lennox

SOB

SOC

MRS Medium

  • 10g/L Peptone
  • 5g/L Yeast Extract
  • 5g/L Sodium Acetate
  • 2g/L Dipotassium Phosphate
  • 2g/l Ammonium Citrate
  • 1g/L Tween-80 (1ml polysorbate-80)
  • 0.1g/L Magnesium Sulfate
  • 0.05g/L Manganese Sulfate (137μL 38% Manganous Sulfate solution)
  • Carbon source:
    • For Full strength medium add 20g/L dextrose and 10g/L beef extract.
    • For other purposes as little as 1.8g/L (10mM) dextrose can be used.
      • If adjusting the pH with malate or arginine use 2.5g/L dextrose.

M17

YPD

RMGX

Personal tools