Richard Lab:PCR: Difference between revisions
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===Overview=== | ===Overview=== | ||
While PCR is typically used for amplification of DNA it can also be used for many other things. Here is a list of the PCR protocols typically used in the Richard Lab. | While PCR is typically used for amplification of DNA it can also be used for many other things. Here is a list of the PCR protocols typically used in the Richard Lab. | ||
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*[[PCR Overlap Extension]] for larger constructs from small parts. | *[[PCR Overlap Extension]] for larger constructs from small parts. | ||
===Mutagenesis=== | ===Mutagenesis=== | ||
*[[Richard Lab:Site Directed Mutagenesis]] | *[[Site-directed mutagenesis|Richard Lab:Site Directed Mutagenesis]] | ||
*Random Mutagenesis | |||
*Saturation Mutagenesis | |||
===Quantification=== | ===Quantification=== |
Revision as of 15:25, 14 September 2011
Back to Richard Lab:protocols
Overview
While PCR is typically used for amplification of DNA it can also be used for many other things. Here is a list of the PCR protocols typically used in the Richard Lab.
Amplification
DNA Synthesis
- DNA Synthesis from Oligos for making smaller parts
- PCR Overlap Extension for larger constructs from small parts.
Mutagenesis
- Richard Lab:Site Directed Mutagenesis
- Random Mutagenesis
- Saturation Mutagenesis