Richard Lab:PCR

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Overview

While PCR is typically used for amplification of DNA it can also be used for many other things. Here is a list of the PCR protocols typically used in the Richard Lab.

Amplification

• For Amplified Insert Assembly
• With Taq Polymerase
• With Vent Polymerase
• With Phusion Polymerase

DNA Synthesis

• DNA Synthesis from Oligos for making smaller parts
• PCR Overlap Extension for larger constructs from small parts.

Mutagenesis

• Richard Lab:Site Directed Mutagenesis
• Random Mutagenesis
• Saturation Mutagenesis

Quantification

• mRNA Quantification by qPCR
• Population Analysis by qPCR