Richard Lab:PCR: Difference between revisions
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===Overview=== | |||
While PCR is typically used for amplification of DNA it can also be used for many other things. Here is a list of the PCR protocols typically used in the Richard Lab. | |||
===Amplification=== | |||
*For Amplified Insert Assembly | |||
*With Taq Polymerase | |||
*With Vent Polymerase | |||
*With Phusion Polymerase | |||
===DNA Synthesis=== | ===DNA Synthesis=== | ||
*[[DNA Synthesis from Oligos]] for making smaller parts | |||
*[[PCR Overlap Extension]] for larger constructs from | *[[PCR Overlap Extension]] for larger constructs from small parts. | ||
===Mutagenesis=== | |||
*[[Site-directed mutagenesis|Richard Lab:Site Directed Mutagenesis]] | |||
*Random Mutagenesis | |||
*Saturation Mutagenesis | |||
===Quantification=== | |||
*[[Richard Lab:mRNA quantification|mRNA Quantification by qPCR]] | |||
*Population Analysis by [[Richard Lab:qPCR|qPCR]] | |||
Latest revision as of 11:57, 26 October 2011
Back to Richard Lab:protocols
Overview
While PCR is typically used for amplification of DNA it can also be used for many other things. Here is a list of the PCR protocols typically used in the Richard Lab.
Amplification
- For Amplified Insert Assembly
- With Taq Polymerase
- With Vent Polymerase
- With Phusion Polymerase
DNA Synthesis
- DNA Synthesis from Oligos for making smaller parts
- PCR Overlap Extension for larger constructs from small parts.
Mutagenesis
- Richard Lab:Site Directed Mutagenesis
- Random Mutagenesis
- Saturation Mutagenesis
Quantification
- mRNA Quantification by qPCR
- Population Analysis by qPCR
