Richard Lab:protocols

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Equipment

Dionex IC

Overview

Eluent Preparation

Standard Preparation

Dionex ASE

Overview

Anaerobic Chamber

Methods

Molecular/Synthetic Biology

Plasmid Extraction

DNA Quantification

DNA Concentration

DNA Synthesis from Oligos

Bio-Brick Digest

Partial Restriction Digest

Agarose Gel Electrophoresis

Polyacrylamide Gel Electrophoresis

Gel Extraction of DNA

Ligation

Preparation of Shuttle-vectors for transformation

Making Electro-competent E. coli

Electro-transformation of E. coli

Making Electro-competent L. plantarum

Electro-transformation of L. Plantarum

Promoter Probing

Chromosomal Integration

Biomass Composition Analysis

NDF (Neutral Detergent Fiber)

ADF (Acid Detergent Fiber)

ADL (Acid Detergent Lignin)

Acetyl Bromide Lignin Analysis

TMAH Lignin Analysis

Manure Storage and Emissions Analysis

Measuring and Analyzing Gas-Flux from a Surface

Setting Up Biofilters in the Field.

Formatting Data from Data Logger

• The following is a set of instructions on how to use macros to transform a data set from configuration 1 to configuration 2.

BOD, moisture and VS content

• Organic matter samples are analyzed according to the following instructions for BOD (Biological Oxygen Demand) and moisture and Volatile Solids (organic matter) content analysis.

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