Richard Lab:protocols

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Equipment

Dionex IC

Overview

Eluent Preparation

Standard Preparation

Dionex ASE

Overview

Anaerobic Chamber

Methods

Molecular/Synthetic Biology

Bio-Brick Formats

Richard Lab PCR Protocols

Bio-Brick Assembly Schedule

Plasmid Extraction

DNA Quantification

DNA Concentration

DNA Synthesis

Restriction Digestion

Partial Restriction Digest

Agarose Gel Electrophoresis

Polyacrylamide Gel Electrophoresis

Gel Extraction of DNA

Ligation

Preparation of Shuttle-vectors for transformation

Making Electro-competent E. coli

Electro-transformation of E. coli

Making Electro-competent L. plantarum

Electro-transformation of L. Plantarum

Chromosomal Integration

Enzymatic Screens and Assays

Ferulic-Acid Esterase Screen

Ferulic Acid Esterase Assay

Laccase Plate Screen

Laccase Assay

Xylanase Plate Screen

Xylanase Assay

Biomass Analysis

Mini-Silo Sampling

NDF (Neutral Detergent Fiber)

ADF (Acid Detergent Fiber)

ADL (Acid Detergent Lignin)

Acetyl Bromide Lignin Analysis

TMAH Lignin Analysis

Enzymatic Hydrolysis of Biomass

Quantification of Glucose

Manure Storage and Emissions Analysis

Measuring and Analyzing Gas-Flux from a Surface

Setting Up Biofilters in the Field.

Formatting Data from Data Logger

• The following is a set of instructions on how to use macros to transform a data set from configuration 1 to configuration 2.

BOD, moisture and VS content

• Organic matter samples are analyzed according to the following instructions for BOD (Biological Oxygen Demand) and moisture and Volatile Solids (organic matter) content analysis.

Compost Analysis

Manure and Compost Sample Preparation

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