Richard Lab:protocols

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(Biomass Analysis)
(Molecular/Synthetic Biology)
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==Molecular/Synthetic Biology==
==Molecular/Synthetic Biology==
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::'''[[Richard Lab:New Standard Assembly| New Standard Assembly]]'''
::'''[[Richard Lab:Bio-Brick Formats|Bio-Brick Formats]]'''
::'''[[Richard Lab:Bio-Brick Formats|Bio-Brick Formats]]'''
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::'''[[Richard Lab:Site Directed Mutagenesis| Site Directed Mutagenesis]]'''
::'''[[Richard Lab:Site Directed Mutagenesis| Site Directed Mutagenesis]]'''
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::'''[[Richard Lab:Bio-Brick Assembly Schedule| New Standard Assembly]]'''
 
::'''[[Miniprep/GET_buffer | Plasmid Extraction]]'''
::'''[[Miniprep/GET_buffer | Plasmid Extraction]]'''

Revision as of 10:40, 4 February 2011

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Contents

Equipment

Dionex IC

Overview
Eluent Preparation
Standard Preparation

Dionex ASE

Overview

Anaerobic Chamber

Methods

Molecular/Synthetic Biology

New Standard Assembly
Bio-Brick Formats
Richard Lab PCR Protocols
Site Directed Mutagenesis
Plasmid Extraction
DNA Quantification
DNA Concentration
DNA Synthesis
Restriction Digestion
Partial Restriction Digest
Agarose Gel Electrophoresis
Polyacrylamide Gel Electrophoresis
Gel Extraction of DNA
Ligation
Preparation of Shuttle-vectors for transformation
Making Electro-competent E. coli
Electro-transformation of E. coli
Making Electro-competent L. plantarum
Electro-transformation of L. Plantarum
Chromosomal Integration

Enzymatic Screens and Assays

Ferulic Acid Esterase Protocols
Laccase Protocols
Xylanase Plate Screen
Xylanase Assay

Biomass Analysis

Mini-Silo Sampling
NDF (Neutral Detergent Fiber)
ADF (Acid Detergent Fiber)
ADL (Acid Detergent Lignin)
Acetyl Bromide Lignin Analysis
TMAH Lignin Analysis
Enzymatic Hydrolysis of Biomass
Quantification of Glucose

Manure Storage and Emissions Analysis

Measuring and Analyzing Gas-Flux from a Surface
Setting Up Biofilters in the Field.
Formatting Data from Data Logger
BOD, moisture and VS content


Compost Analysis

Manure and Compost Sample Preparation
Chemical Oxygen Demand

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