Richard Lab:protocols

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(Enzymatic Screens and Assays)
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=Methods=
=Methods=
</center>
</center>
 +
==Biomass Analysis==
 +
 +
::'''[[Mini-Silo Sampling|Mini-Silo Sampling]]'''
 +
::'''[[Richard_Lab:_NDF|NDF (Neutral Detergent Fiber)]]'''
 +
::'''[[Richard_Lab:ADF|ADF (Acid Detergent Fiber)]]'''
 +
::'''[[Richard_Lab:ADL|ADL (Acid Detergent Lignin)]]'''
 +
::'''Acetyl Bromide Lignin Analysis'''
 +
::'''TMAH Lignin Analysis'''
 +
::'''[[Richard Lab:Enzymatic Hydrolysis of Biomass|Enzymatic Hydrolysis of Biomass]]'''
 +
::'''Quantification of Glucose'''
 +
 +
==Enzymatic Screens and Assays==
 +
 +
::'''[[Feruloyl Esterase Protocols|Ferulic Acid Esterase Protocols]]'''
 +
::'''[[Laccase Protocols]]'''
 +
::'''Xylanase Protocols'''
 +
::'''[[Beta-galactosidase assay|Beta-galactosidase (LacZ) Assay]]'''
 +
::'''[[Beta-galactosidase Screen]]
 +
==Molecular/Synthetic Biology==
==Molecular/Synthetic Biology==
::'''[[Richard Lab:Amplified Insert Assembly|Amplified Insert Assembly]]'''
::'''[[Richard Lab:Amplified Insert Assembly|Amplified Insert Assembly]]'''
-
 
::'''[[Richard Lab:Bio-Brick Formats|Bio-Brick Formats]]'''
::'''[[Richard Lab:Bio-Brick Formats|Bio-Brick Formats]]'''
-
 
::'''Richard Lab PCR Protocols'''
::'''Richard Lab PCR Protocols'''
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::'''[[Richard Lab:Site Directed Mutagenesis| Site Directed Mutagenesis]]'''
::'''[[Richard Lab:Site Directed Mutagenesis| Site Directed Mutagenesis]]'''
-
 
::'''[[Miniprep/GET_buffer | Plasmid Extraction]]'''
::'''[[Miniprep/GET_buffer | Plasmid Extraction]]'''
-
 
::'''[[DNA Quantification]]'''
::'''[[DNA Quantification]]'''
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::'''[[Ethanol_precipitation_of_nucleic_acids | DNA Concentration]]'''
::'''[[Ethanol_precipitation_of_nucleic_acids | DNA Concentration]]'''
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::'''[[DNA Synthesis from Oligos|DNA Synthesis]]'''
::'''[[DNA Synthesis from Oligos|DNA Synthesis]]'''
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::'''[[Richard_Lab:Restriction_Digest | Restriction Digestion]]'''
::'''[[Richard_Lab:Restriction_Digest | Restriction Digestion]]'''
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::'''[[Partial Restriction Digest]]'''
::'''[[Partial Restriction Digest]]'''
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::'''[[Richard_Lab:_Agarose_Gel_Electrophoresis| Agarose Gel Electrophoresis]]'''
::'''[[Richard_Lab:_Agarose_Gel_Electrophoresis| Agarose Gel Electrophoresis]]'''
-
 
::'''[[Polyacrylamide Gel Electrophoresis]]'''
::'''[[Polyacrylamide Gel Electrophoresis]]'''
-
 
::'''[[DNA_Gel_extraction | Gel Extraction of DNA]]'''
::'''[[DNA_Gel_extraction | Gel Extraction of DNA]]'''
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::'''[[Richard_Lab:Ligation|Ligation]]'''
::'''[[Richard_Lab:Ligation|Ligation]]'''
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::'''[[In vitro modification of DNA for L. plantarum|Preparation of Shuttle-vectors for transformation]]'''
::'''[[In vitro modification of DNA for L. plantarum|Preparation of Shuttle-vectors for transformation]]'''
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::'''[[Richard_Lab:Preparing_electrocompetent_cells|Making Electro-competent ''E. coli'']]'''
::'''[[Richard_Lab:Preparing_electrocompetent_cells|Making Electro-competent ''E. coli'']]'''
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::'''[[Richard Lab:Electroporation of E. coli|Electro-transformation of ''E. coli'']]'''
::'''[[Richard Lab:Electroporation of E. coli|Electro-transformation of ''E. coli'']]'''
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::'''[[Preparation of Lactobacillus Competent Cells|Making Electro-competent ''L. plantarum'']]'''
::'''[[Preparation of Lactobacillus Competent Cells|Making Electro-competent ''L. plantarum'']]'''
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::'''[[Electrotransformation of Lactobacillus plantarum|Electro-transformation of ''L. Plantarum'']]'''
::'''[[Electrotransformation of Lactobacillus plantarum|Electro-transformation of ''L. Plantarum'']]'''
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::'''Chromosomal Integration'''
::'''Chromosomal Integration'''
-
 
-
==Enzymatic Screens and Assays==
 
-
::'''[[Feruloyl Esterase Protocols|Ferulic Acid Esterase Protocols]]'''
 
-
::'''[[Laccase Protocols]]'''
 
-
::'''Xylanase Protocols'''
 
-
::'''[[Beta-galactosidase assay|Beta-galactosidase (LacZ) Assay]]'''
 
-
::'''[[Beta-galactosidase Screen]]
 
-
 
-
==Biomass Analysis==
 
-
 
-
::'''[[Mini-Silo Sampling|Mini-Silo Sampling]]'''
 
-
 
-
::'''[[Richard_Lab:_NDF|NDF (Neutral Detergent Fiber)]]'''
 
-
 
-
::'''[[Richard_Lab:ADF|ADF (Acid Detergent Fiber)]]'''
 
-
 
-
::'''[[Richard_Lab:ADL|ADL (Acid Detergent Lignin)]]'''
 
-
 
-
::'''Acetyl Bromide Lignin Analysis'''
 
-
 
-
::'''TMAH Lignin Analysis'''
 
-
 
-
::'''[[Richard Lab:Enzymatic Hydrolysis of Biomass|Enzymatic Hydrolysis of Biomass]]'''
 
-
 
-
::'''Quantification of Glucose'''
 
==Manure Storage and Emissions Analysis==
==Manure Storage and Emissions Analysis==
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::'''BOD, moisture and VS content'''
::'''BOD, moisture and VS content'''
:::*Organic matter samples are analyzed according to the following instructions for [http://www.abe.psu.edu/bioconversion/biofilter_characterization/BOD.htm BOD (Biological Oxygen Demand)] and [http://www.abe.psu.edu/bioconversion/biofilter_characterization/moisture_and_VS.htm moisture and Volatile Solids (organic matter) content] analysis.
:::*Organic matter samples are analyzed according to the following instructions for [http://www.abe.psu.edu/bioconversion/biofilter_characterization/BOD.htm BOD (Biological Oxygen Demand)] and [http://www.abe.psu.edu/bioconversion/biofilter_characterization/moisture_and_VS.htm moisture and Volatile Solids (organic matter) content] analysis.
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==Compost Analysis==
==Compost Analysis==

Revision as of 16:49, 22 March 2011

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Contents

Equipment

Dionex IC

Overview
Eluent Preparation
Standard Preparation

Dionex ASE

Overview

Anaerobic Chamber

Methods

Biomass Analysis

Mini-Silo Sampling
NDF (Neutral Detergent Fiber)
ADF (Acid Detergent Fiber)
ADL (Acid Detergent Lignin)
Acetyl Bromide Lignin Analysis
TMAH Lignin Analysis
Enzymatic Hydrolysis of Biomass
Quantification of Glucose

Enzymatic Screens and Assays

Ferulic Acid Esterase Protocols
Laccase Protocols
Xylanase Protocols
Beta-galactosidase (LacZ) Assay
Beta-galactosidase Screen

Molecular/Synthetic Biology

Amplified Insert Assembly
Bio-Brick Formats
Richard Lab PCR Protocols
Site Directed Mutagenesis
Plasmid Extraction
DNA Quantification
DNA Concentration
DNA Synthesis
Restriction Digestion
Partial Restriction Digest
Agarose Gel Electrophoresis
Polyacrylamide Gel Electrophoresis
Gel Extraction of DNA
Ligation
Preparation of Shuttle-vectors for transformation
Making Electro-competent E. coli
Electro-transformation of E. coli
Making Electro-competent L. plantarum
Electro-transformation of L. Plantarum
Chromosomal Integration

Manure Storage and Emissions Analysis

Measuring and Analyzing Gas-Flux from a Surface
Setting Up Biofilters in the Field.
Formatting Data from Data Logger
BOD, moisture and VS content

Compost Analysis

Manure and Compost Sample Preparation
Chemical Oxygen Demand

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