Richard Lab:protocols

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(Enzymatic Screens and Assays)
(Biomass Analysis)
Line 16: Line 16:
==Biomass Analysis==
==Biomass Analysis==
::'''[[Silage protocols]]'''
::'''[[Mini-Silo Sampling|Mini-Silo Sampling]]'''
::'''[[Mini-Silo Sampling|Mini-Silo Sampling]]'''
::'''[[Richard_Lab:_NDF|NDF (Neutral Detergent Fiber)]]'''
::'''[[Richard_Lab:_NDF|NDF (Neutral Detergent Fiber)]]'''

Revision as of 19:25, 8 June 2011

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Dionex IC

Eluent Preparation
Standard Preparation

Dionex ASE


Anaerobic Chamber


Biomass Analysis

Silage protocols
Mini-Silo Sampling
NDF (Neutral Detergent Fiber)
ADF (Acid Detergent Fiber)
ADL (Acid Detergent Lignin)
Acetyl Bromide Lignin Analysis
TMAH Lignin Analysis
Enzymatic Hydrolysis of Biomass
Quantification of Glucose

Enzymatic Screens and Assays

Ferulic Acid Esterase Protocols
Laccase Protocols
Xylanase Protocols
Beta-galactosidase (LacZ) Assay
Beta-galactosidase Screen

Molecular/Synthetic Biology

Amplified Insert Assembly
Bio-Brick Formats
Richard Lab PCR Protocols
Site Directed Mutagenesis
Plasmid Extraction
DNA Quantification
DNA Concentration
DNA Synthesis
Restriction Digestion
Partial Restriction Digest
Agarose Gel Electrophoresis
Polyacrylamide Gel Electrophoresis
Gel Extraction of DNA
Preparation of Shuttle-vectors for transformation
Transforming Lactococcus lactis
Making Electro-competent E. coli
Electro-transformation of E. coli
Making Electro-competent L. plantarum
Electro-transformation of L. Plantarum
Chromosomal Integration

Manure Storage and Emissions Analysis

Measuring and Analyzing Gas-Flux from a Surface
Setting Up Biofilters in the Field.
Formatting Data from Data Logger
BOD, moisture and VS content

Compost Analysis

Manure and Compost Sample Preparation
Chemical Oxygen Demand

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