Richard Lab:protocols

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Biomass Analysis)
(Biomass Analysis)
Line 18: Line 18:
=Methods=
=Methods=
</center>
</center>
-
==Biomass Analysis==
+
==Biomass Protocols==
::'''[[Silage protocols|Silage Protocols]]'''
::'''[[Silage protocols|Silage Protocols]]'''
Line 28: Line 28:
::'''[[Richard Lab:Enzymatic Hydrolysis of Biomass|Enzymatic Hydrolysis of Biomass]]'''
::'''[[Richard Lab:Enzymatic Hydrolysis of Biomass|Enzymatic Hydrolysis of Biomass]]'''
::'''[[Quantification of glucose|Quantification of Glucose]]'''
::'''[[Quantification of glucose|Quantification of Glucose]]'''
 +
::'''[[Richard Lab:SSCF|Simultaneous Saccharification and Co-Fermentation]]'''
==Enzymatic Screens and Assays==
==Enzymatic Screens and Assays==

Revision as of 15:31, 12 September 2011

Back to Richard Lab Main Page

Contents

Equipment

Dionex IC

Overview
Eluent Preparation
Standard Preparation

Dionex ASE

Overview

Anaerobic Chamber

Overview

YSI Analyzer

Overview

Methods

Biomass Protocols

Silage Protocols
Biomass Compositional Analysis
NDF (Neutral Detergent Fiber) Determination
ADF (Acid Detergent Fiber) Determination
ADL (Acid Detergent Lignin) Determination
Dilute-Acid Pretreatment of Biomass
Enzymatic Hydrolysis of Biomass
Quantification of Glucose
Simultaneous Saccharification and Co-Fermentation

Enzymatic Screens and Assays

Ferulic Acid Esterase Protocols
Laccase Protocols
Xylanase Protocols
Beta-galactosidase (LacZ) Assay
Beta-galactosidase Screen
Beta-glucuronidase (GusA) Protocols

Molecular/Synthetic Biology

Amplified Insert Assembly
Bio-Brick Formats
Richard Lab PCR Protocols
Site Directed Mutagenesis
Plasmid Extraction
DNA Quantification
DNA Concentration
DNA Synthesis
Restriction Digestion
Partial Restriction Digest
Agarose Gel Electrophoresis
Polyacrylamide Gel Electrophoresis
Gel Extraction of DNA
Ligation
Preparation of Shuttle-vectors for transformation
Transforming Lactococcus lactis
Making Electro-competent E. coli
Electro-transformation of E. coli
Electro-transformation of L. Plantarum
Chromosomal Integration

Manure Storage and Emissions Analysis

Measuring and Analyzing Gas-Flux from a Surface
Setting Up Biofilters in the Field.
Formatting Data from Data Logger
BOD, moisture and VS content

Compost Analysis

Manure and Compost Sample Preparation
Chemical Oxygen Demand

Back to Richard Lab

Personal tools