Richard Lab:protocols

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Equipment

Dionex IC

Overview

Eluent Preparation

Standard Preparation

Dionex ASE

Overview

Anaerobic Chamber

• Put protocol here

Data Logger

To Reconfigure data into a compatible format

• The following is a set of instructions on how to use macros to transform a data set from configuration 1 to configuration 2.

Methods

Molecular/Synthetic Biology

Plasmid Extraction

DNA Quantification

DNA Concentration

DNA Synthesis from Oligos

Bio-Brick Digest

Partial Restriction Digest

Agarose Gel Electrophoresis

Polyacrylamide Gel Electrophoresis

Gel Extraction of DNA

Ligation

Electro-transformation of E. coli

Electro-transformation of Lactobacillus

Making Electro-competent Cells

Promoter Probing

Fiber Analysis

NDF (Neutral Detergent Fiber)

ADF (Acid Detergent Fiber)

ADL (Acid Detergent Lignin)

TMAH Lignin Analysis

BOD, moisture and VS content

• Organic matter samples are analyzed according to the following instructions for BOD (Biological Oxygen Demand) and moisture and Volatile Solids (organic matter) content analysis.

Manure Storage and Emissions Analysis

Measuring and Analyzing Gas-Flux from a Surface

• gaseous emission data is collected using a static flux chamber and a photoacoustic sensor.
• an Excel program is then used to extract relevant data (from the continuous data collected by the photoacoustic sensor every minute). The same program also calculates the flux of selected gases from the tested surface, using Pedersen's equation (2001).
• selected data are copied and pasted as values into a compiled data set
• a regression analysis is performed to evaluate the effect of time, biofilter treatment, and other input variables on the flux level of each analyzed gas: ammonia, methane, carbon dioxide, nitrous oxide and water vapor. The Sagata Regression Pro software (ltd) is used: go to regression > pick inputs > pick outputs > chose data types (categorical or continuous) > chose regression model (to evaluate interactions create input variables made of factors evaluate for interaction amongst them, multiplied by each other) > go to output, get response vs. factors for preliminary graphs > choose indicative title for the output sheet > click ok.
• the statistical analysis output is interpreted: the first Anova table shows possible treatment effect. The "term significance" table shows effect of individual factors (or interactions).

Method used to set up biofilters on a surface in the field

How biofilters are set up.

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