Richard Lab:protocols

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 52: Line 52:
::'''[[Richard_Lab:_NDF|NDF (Neutral Detergent Fiber)]]'''
::'''[[Richard_Lab:_NDF|NDF (Neutral Detergent Fiber)]]'''
-
::'''[[Richard_Lab:ADF|ADF (Acid Detergent Fiber)]]'''
+
::'''[[ADF|ADF (Acid Detergent Fiber)]]'''
-
::'''[[ADL]]'''
+
::'''[[Richard_Lab:ADL|ADL (Acid Detergent Lignin)]]'''
::'''TMAH Lignin Analysis'''
::'''TMAH Lignin Analysis'''

Revision as of 14:22, 3 February 2010

Back to Richard Lab

Contents

Equipment

Dionex IC

Overview
Eluent Preparation
Standard Preparation

Dionex ASE

Overview

Anaerobic Chamber

  • Put protocol here

Data Logger

To Reconfigure data into a compatible format

Methods

Molecular/Synthetic Biology

Plasmid Extraction
DNA Quantification
DNA Concentration
DNA Synthesis from Oligos
Bio-Brick Digest
Partial Restriction Digest
Agarose Gel Electrophoresis
Polyacrylamide Gel Electrophoresis
Gel Extraction of DNA
Ligation
Electro-transformation of E. coli
Electro-transformation of Lactobacillus
Making Electro-competent Cells
Promoter Probing

Fiber Analysis

NDF (Neutral Detergent Fiber)
ADF (Acid Detergent Fiber)
ADL (Acid Detergent Lignin)
TMAH Lignin Analysis
BOD, moisture and VS content

Manure Storage and Emissions Analysis

Measuring and Analyzing Gas-Flux from a Surface

  • gaseous emission data is collected using a static flux chamber and a photoacoustic sensor.
  • an Excel program is then used to extract relevant data (from the continuous data collected by the photoacoustic sensor every minute). The same program also calculates the flux of selected gases from the tested surface, using Pedersen's equation (2001).
  • selected data are copied and pasted as values into a compiled data set
  • a regression analysis is performed to evaluate the effect of time, biofilter treatment, and other input variables on the flux level of each analyzed gas: ammonia, methane, carbon dioxide, nitrous oxide and water vapor. The Sagata Regression Pro software (ltd) is used: go to regression > pick inputs > pick outputs > chose data types (categorical or continuous) > chose regression model (to evaluate interactions create input variables made of factors evaluate for interaction amongst them, multiplied by each other) > go to output, get response vs. factors for preliminary graphs > choose indicative title for the output sheet > click ok.
  • the statistical analysis output is interpreted: the first Anova table shows possible treatment effect. The "term significance" table shows effect of individual factors (or interactions).

Method used to set up biofilters on a surface in the field

How biofilters are set up.

Back to Richard Lab

Personal tools