Richard Lab:protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
| Line 61: | Line 61: | ||
==Manure Storage and Emissions Analysis== | ==Manure Storage and Emissions Analysis== | ||
::'''[[Richard Lab:Gas_Flux|Measuring and Analyzing Gas-Flux from a Surface]]''' | |||
*gaseous emission data is collected using a [http://www.abe.psu.edu/bioconversion/gas_sampling.ppt static flux chamber and a photoacoustic sensor]. | *gaseous emission data is collected using a [http://www.abe.psu.edu/bioconversion/gas_sampling.ppt static flux chamber and a photoacoustic sensor]. | ||
*an [http://www.abe.psu.edu/bioconversion/programPAS.xls Excel program] is then used to extract relevant data (from the continuous data collected by the photoacoustic sensor every minute). The same program also calculates the flux of selected gases from the tested surface, using Pedersen's equation (2001). | *an [http://www.abe.psu.edu/bioconversion/programPAS.xls Excel program] is then used to extract relevant data (from the continuous data collected by the photoacoustic sensor every minute). The same program also calculates the flux of selected gases from the tested surface, using Pedersen's equation (2001). | ||
Revision as of 16:07, 9 February 2010
Back to Richard Lab
Equipment
Dionex IC
Dionex ASE
Anaerobic Chamber
Methods
Molecular/Synthetic Biology
- Electro-transformation of Lactobacillus
- Promoter Probing
Biomass Composition Analysis
- Acetyl Bromide Lignin Analysis
- TMAH Lignin Analysis
- BOD, moisture and VS content
- Organic matter samples are analyzed according to the following instructions for BOD (Biological Oxygen Demand) and moisture and Volatile Solids (organic matter) content analysis.
- BOD, moisture and VS content
Manure Storage and Emissions Analysis
- gaseous emission data is collected using a static flux chamber and a photoacoustic sensor.
- an Excel program is then used to extract relevant data (from the continuous data collected by the photoacoustic sensor every minute). The same program also calculates the flux of selected gases from the tested surface, using Pedersen's equation (2001).
- selected data are copied and pasted as values into a compiled data set
- a regression analysis is performed to evaluate the effect of time, biofilter treatment, and other input variables on the flux level of each analyzed gas: ammonia, methane, carbon dioxide, nitrous oxide and water vapor. The Sagata Regression Pro software (ltd) is used: go to regression > pick inputs > pick outputs > chose data types (categorical or continuous) > chose regression model (to evaluate interactions create input variables made of factors evaluate for interaction amongst them, multiplied by each other) > go to output, get response vs. factors for preliminary graphs > choose indicative title for the output sheet > click ok.
- the statistical analysis output is interpreted: the first Anova table shows possible treatment effect. The "term significance" table shows effect of individual factors (or interactions).
Method used to set up biofilters on a surface in the field
To Reconfigure data from data logger into a compatible format
- The following is a set of instructions on how to use macros to transform a data set from configuration 1 to configuration 2.
Back to Richard Lab
