Richard Lab:protocols

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(Biomass Composition Analysis)
(Manure Storage and Emissions Analysis)
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::'''[http://www.abe.psu.edu/bioconversion/biofilters_setup.ppt Setting Up Biofilters in the Field].
::'''[http://www.abe.psu.edu/bioconversion/biofilters_setup.ppt Setting Up Biofilters in the Field].
::'''Formatting Data from Data Logger'''
::'''Formatting Data from Data Logger'''
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====To Reconfigure data from data logger into a compatible format====
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:::*The following is a set of [http://www.abe.psu.edu/bioconversion/temperature/instructions.htm instructions on how to use macros] to transform a data set from [http://www.abe.psu.edu/bioconversion/temperature/config1.htm configuration 1] to [http://www.abe.psu.edu/bioconversion/temperature/config2.htm configuration 2].
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*The following is a set of [http://www.abe.psu.edu/bioconversion/temperature/instructions.htm instructions on how to use macros] to transform a data set from [http://www.abe.psu.edu/bioconversion/temperature/config1.htm configuration 1] to [http://www.abe.psu.edu/bioconversion/temperature/config2.htm configuration 2].
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::'''BOD, moisture and VS content'''
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:::*Organic matter samples are analyzed according to the following instructions for [http://www.abe.psu.edu/bioconversion/biofilter_characterization/BOD.htm BOD (Biological Oxygen Demand)] and [http://www.abe.psu.edu/bioconversion/biofilter_characterization/moisture_and_VS.htm moisture and Volatile Solids (organic matter) content] analysis.
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Revision as of 19:14, 9 February 2010

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Contents

Equipment

Dionex IC

Overview
Eluent Preparation
Standard Preparation

Dionex ASE

Overview

Anaerobic Chamber

Methods

Molecular/Synthetic Biology

Plasmid Extraction
DNA Quantification
DNA Concentration
DNA Synthesis from Oligos
Bio-Brick Digest
Partial Restriction Digest
Agarose Gel Electrophoresis
Polyacrylamide Gel Electrophoresis
Gel Extraction of DNA
Ligation
Electro-transformation of E. coli
Electro-transformation of Lactobacillus
Making Electro-competent Cells
Promoter Probing

Biomass Composition Analysis

NDF (Neutral Detergent Fiber)
ADF (Acid Detergent Fiber)
ADL (Acid Detergent Lignin)
Acetyl Bromide Lignin Analysis
TMAH Lignin Analysis

Manure Storage and Emissions Analysis

Measuring and Analyzing Gas-Flux from a Surface
Setting Up Biofilters in the Field.
Formatting Data from Data Logger
BOD, moisture and VS content

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