Richard Lab:protocols

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(Enzymatic Screens and Assays)
(Compost Analysis)
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==Compost Analysis==
==Compost Analysis==
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insert method links here
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[[Manure and Compost Sample Preparation|other name Manure and Compost Sample Preparation]]
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Back to [[Richard_Lab | Richard Lab]]
Back to [[Richard_Lab | Richard Lab]]

Revision as of 18:33, 13 January 2011

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Contents

Equipment

Dionex IC

Overview
Eluent Preparation
Standard Preparation

Dionex ASE

Overview

Anaerobic Chamber

Methods

Molecular/Synthetic Biology

Richard Lab Bio-Brick Formats
Richard Lab PCR Protocols
Bio-Brick Assembly Schedule
Plasmid Extraction
DNA Quantification
DNA Concentration
DNA Synthesis
Restriction Digestion
Partial Restriction Digest
Agarose Gel Electrophoresis
Polyacrylamide Gel Electrophoresis
Gel Extraction of DNA
Ligation
Preparation of Shuttle-vectors for transformation
Making Electro-competent E. coli
Electro-transformation of E. coli
Making Electro-competent L. plantarum
Electro-transformation of L. Plantarum
Chromosomal Integration

Enzymatic Screens and Assays

Ferulic-Acid Esterase Plate Screen
Ferulic Acid Esterase Assay
Laccase Plate Screen
Laccase Assay
Xylanase Plate Screen
Xylanase Assay

Biomass Composition Analysis

NDF (Neutral Detergent Fiber)
ADF (Acid Detergent Fiber)
ADL (Acid Detergent Lignin)
Acetyl Bromide Lignin Analysis
TMAH Lignin Analysis
Enzymatic Hydrolysis of Biomass
Quantification of glucose using the YSI

Manure Storage and Emissions Analysis

Measuring and Analyzing Gas-Flux from a Surface
Setting Up Biofilters in the Field.
Formatting Data from Data Logger
BOD, moisture and VS content

Compost Analysis

other name Manure and Compost Sample Preparation

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