Richard Lab:protocols: Difference between revisions
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::'''[[DNA_Gel_extraction | Gel Extraction of DNA]]''' | ::'''[[DNA_Gel_extraction | Gel Extraction of DNA]]''' | ||
::'''[[Richard_Lab:Ligation|Ligation]]''' | ::'''[[Richard_Lab:Ligation|Ligation]]''' | ||
::'''[[In vitro modification of DNA for L. plantarum|Preparation of Shuttle-vectors for transformation]]'' | ::'''[[In vitro modification of DNA for L. plantarum|Preparation of Shuttle-vectors for transformation]]''' | ||
::'''[[Transforming Lactococcus lactis]]''' | ::'''[[Transforming Lactococcus lactis]]''' | ||
::'''[[Richard_Lab:Preparing_electrocompetent_cells|Making Electro-competent ''E. coli'']]''' | ::'''[[Richard_Lab:Preparing_electrocompetent_cells|Making Electro-competent ''E. coli'']]''' | ||
Revision as of 15:54, 5 April 2011
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Equipment
Dionex IC
Dionex ASE
Anaerobic Chamber
Methods
Biomass Analysis
- Mini-Silo Sampling
- NDF (Neutral Detergent Fiber)
- ADF (Acid Detergent Fiber)
- ADL (Acid Detergent Lignin)
- Acetyl Bromide Lignin Analysis
- TMAH Lignin Analysis
- Enzymatic Hydrolysis of Biomass
- Quantification of Glucose
Enzymatic Screens and Assays
Molecular/Synthetic Biology
- Amplified Insert Assembly
- Bio-Brick Formats
- Richard Lab PCR Protocols
- Site Directed Mutagenesis
- Plasmid Extraction
- DNA Quantification
- DNA Concentration
- DNA Synthesis
- Restriction Digestion
- Partial Restriction Digest
- Agarose Gel Electrophoresis
- Polyacrylamide Gel Electrophoresis
- Gel Extraction of DNA
- Ligation
- Preparation of Shuttle-vectors for transformation
- Transforming Lactococcus lactis
- Making Electro-competent E. coli
- Electro-transformation of E. coli
- Making Electro-competent L. plantarum
- Electro-transformation of L. Plantarum
- Chromosomal Integration
Manure Storage and Emissions Analysis
- Measuring and Analyzing Gas-Flux from a Surface
- Setting Up Biofilters in the Field.
- Formatting Data from Data Logger
- The following is a set of instructions on how to use macros to transform a data set from configuration 1 to configuration 2.
- BOD, moisture and VS content
- Organic matter samples are analyzed according to the following instructions for BOD (Biological Oxygen Demand) and moisture and Volatile Solids (organic matter) content analysis.
Compost Analysis
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