Richard Lab:protocols
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Equipment
Dionex IC
- Put protocol here
Dionex ASE
- Put protocol here
Anaerobic Chamber
- Put protocol here
Data Logger
To Reconfigure data into a compatible format
- The following is a set of instructions on how to use macros to transform a data set from configuration 1 to configuration 2.
Methods
Molecular Biology
Promoter Probing
- Put Protocol here
Transformation using Electroporator
- Put protocol here
Fiber Analysis
NDF
- Put protocol here
ADF
- Put Protocol here
TMAH Lignin Analysis
- Put protocol here
BOD, moisture and VS content
Organic matter samples are analyzed according to the following instructions for BOD (Biological Oxygen Demand) and moisture and Volatile Solids (organic matter) content analysis.
Gas Analysis
Method used to measure and analyze gas fluxes from a given surface
- gaseous emission data is collected using a static flux chamber and a photoacoustic sensor.
- an Excel program is then used to extract relevant data (from the continuous data collected by the photoacoustic sensor every minute). The same program also calculates the flux of selected gases from the tested surface, using Pedersen's equation (2001).
- selected data are copied and pasted as values into a compiled data set
- a regression analysis is performed to evaluate the effect of time, biofilter treatment, and other input variables on the flux level of each analyzed gas: ammonia, methane, carbon dioxide, nitrous oxide and water vapor. The Sagata Regression Pro software (ltd) is used: go to regression > pick inputs > pick outputs > chose data types (categorical or continuous) > chose regression model (to evaluate interactions create input variables made of factors evaluate for interaction amongst them, multiplied by each other) > go to output, get response vs. factors for preliminary graphs > choose indicative title for the output sheet > click ok.
- the statistical analysis output is interpreted: the first Anova table shows possible treatment effect. The "term significance" table shows effect of individual factors (or interactions).
Method used to set up biofilters on a surface in the field
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