Richard Lab:protocols

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Contents

Equipment

Dionex IC

Overview
Eluent Preparation
Standard Preparation

Dionex ASE

Overview

Anaerobic Chamber

YSI Analyzer

Overview

Methods

Biomass Analysis

Silage protocols
Biomass compositional analysis
NDF (Neutral Detergent Fiber) Determination
ADF (Acid Detergent Fiber) Determination
ADL (Acid Detergent Lignin) Determination
Enzymatic Hydrolysis of Biomass
Quantification of Glucose

Enzymatic Screens and Assays

Ferulic Acid Esterase Protocols
Laccase Protocols
Xylanase Protocols
Beta-galactosidase (LacZ) Assay
Beta-galactosidase Screen
Beta-glucuronidase protocols

Molecular/Synthetic Biology

Amplified Insert Assembly
Bio-Brick Formats
Richard Lab PCR Protocols
Site Directed Mutagenesis
Plasmid Extraction
DNA Quantification
DNA Concentration
DNA Synthesis
Restriction Digestion
Partial Restriction Digest
Agarose Gel Electrophoresis
Polyacrylamide Gel Electrophoresis
Gel Extraction of DNA
Ligation
Preparation of Shuttle-vectors for transformation
Transforming Lactococcus lactis
Making Electro-competent E. coli
Electro-transformation of E. coli
Electro-transformation of L. Plantarum
Chromosomal Integration

Manure Storage and Emissions Analysis

Measuring and Analyzing Gas-Flux from a Surface
Setting Up Biofilters in the Field.
Formatting Data from Data Logger
BOD, moisture and VS content

Compost Analysis

Manure and Compost Sample Preparation
Chemical Oxygen Demand

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