Rickus Lab Safety: Difference between revisions

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==EMERGENCY CONTACTS==
==EMERGENCY CONTACTS==
[http://www.purdue.edu/physicalfacilities/emergencycontacts.php Purdue Emergency Contacts]
 
In a real emergency, call 911
 
[https://www.purdue.edu/physicalfacilities/emergcontacts/index.html Case Specific Purdue Emergency Contacts]


Prof. Rickus 494-1197
Prof. Rickus 494-1197
Line 12: Line 15:
[http://www.purdue.edu/rem/eh/bst_files/frame.htm On-line Biological Safety Training]
[http://www.purdue.edu/rem/eh/bst_files/frame.htm On-line Biological Safety Training]


[http://www.purdue.edu/rem/eh/hhcl_files/v3_document.htm Handling Human Cell Lines]
[http://www.purdue.edu/rem/home/files/training.htm Handling Human Cell Lines]


[http://www.purdue.edu/rem/home/booklets/CHP2003.pdf Purdue Chemical Hygiene Plan]
[http://www.purdue.edu/rem/home/booklets/CHP2003.pdf Purdue Chemical Hygiene Plan]
Line 20: Line 23:
[http://www.purdue.edu/rem/laser/laser.htm  Purdue REM Laser Safety]
[http://www.purdue.edu/rem/laser/laser.htm  Purdue REM Laser Safety]


==Initial Safety Requirement for New Lab Members==
[http://www2.itap.purdue.edu/bnc/training/  Birck Nanotechnology Training]
 
[http://www.cdc.gov/biosafety/publications/bmbl5/BMBL5_sect_IV.pdf  CDC Biosafety Publication]
 
==Safety Requirements for All Lab Members==


'''All new lab members must:'''
'''All new lab members must:'''
# Go through the Bindley Bioscience Center new user process (form to be completed, meet with building staff)
# Read the Purdue Chemical Hygiene Plan
# Read the Purdue Chemical Hygiene Plan
# Sign the [http://www.purdue.edu/rem/home/forms/aware.pdf Chemical Hygiene Awareness Certificate].  Turn in to Dr. Rickus
# Sign the [http://www.purdue.edu/rem/home/forms/aware.pdf Chemical Hygiene Awareness Certificate].  Turn in to Dr. Rickus
Line 29: Line 37:
# Take note of the Hazard Assessment Sheet on the door of your work area
# Take note of the Hazard Assessment Sheet on the door of your work area
# Locate the MSDS books in your work area
# Locate the MSDS books in your work area
# Document their training on the wiki Safety Training Log
# Check in with Dr. Rickus to discuss and document the completion of these steps.
 
'''If you will be working in Birck, you must go through Birck Specific Training in Addition to the steps above"'
# [http://www.purdue.edu/discoverypark/nanotechnology/facilities/training.php Click here to sign up for Birck Training]


'''If you will be handling or working around biological samples.  You must'''
'''If you will be handling or working around biological samples.  You must'''
Line 37: Line 48:
# Complete and sign the  [http://www.purdue.edu/rem/home/forms/bsaware.pdf biological safety training certificate].  Turn in the form to Dr. Rickus.
# Complete and sign the  [http://www.purdue.edu/rem/home/forms/bsaware.pdf biological safety training certificate].  Turn in the form to Dr. Rickus.


'''If you will be working with or around human cell lines (such as the Caco-2 cells) you must:'''
'''If you will be working in BSL2 areas of Bindley or Birck, you must'''
# Read the Handling Human Cell Lines presentation
#Complete bloodborne pathogen training.  You can complete this trough REM. [http://www.purdue.edu/rem/eh/bloodpth.htm Click here] for info.  or through Lisa Reese in Birck. [http://www2.itap.purdue.edu/bnc/training/ Click Here] to register
 
'''If you will be working with or around human cell lines (e.g. Caco-2, GAMB1) you must also:'''
# Read the Handling Human Cell Lines presentation [http://www.purdue.edu/rem/home/files/training.htm Handling Human Cell Lines]
# Complete and sign the human cell line certification form located at the end of the training presentation.
# Note that all human cell lines are considered at least BSL2, so you must complete bloodborne pathogen training.


==Special Safety Notes of Particular Relevance to the Rickus Lab==
==Special Safety Notes of Particular Relevance to the Rickus Lab==
Line 54: Line 70:
[http://engineering.tufts.edu/microfab/index_files/SOP/PiranhaClean_SOP.pdf Tufts]
[http://engineering.tufts.edu/microfab/index_files/SOP/PiranhaClean_SOP.pdf Tufts]


==Molecular Biology, Bindley 134==
===Molecular Biology, Bindley 134===
#'''ethidium bromide'''. Ethidium bromide (EtBr), 3,8-diamino-5-ethyl-6-phenylphenanthridinium bromide, is commonly used as a non-radioactive marker for identifying and visualizing nucleic acid bands in electrophoresis and other methods of gel-based nucleic acid separation. EtBr is a dark red crystalline that readily fluoresces reddish-brown when exposed to ultraviolet (UV) light. EtBr is a potent mutagen and toxic after an acute exposure. It is an irritant to the skin, eyes, mouth, and upper respiratory tract. Direct contact with EtBr should be avoided.  Read REM's safety information for [http://www.purdue.edu/rem/hmm/ethidbr.htm proper handling and disposal of ethidium bromide]
#'''ethidium bromide'''. Ethidium bromide (EtBr), 3,8-diamino-5-ethyl-6-phenylphenanthridinium bromide, is commonly used as a non-radioactive marker for identifying and visualizing nucleic acid bands in electrophoresis and other methods of gel-based nucleic acid separation. EtBr is a dark red crystalline that readily fluoresces reddish-brown when exposed to ultraviolet (UV) light. EtBr is a potent mutagen and toxic after an acute exposure. It is an irritant to the skin, eyes, mouth, and upper respiratory tract. Direct contact with EtBr should be avoided.  Read REM's safety information for [http://www.purdue.edu/rem/hmm/ethidbr.htm proper handling and disposal of ethidium bromide]
===BSL2 Cell Culture Lab, 2nd floor===
===BSL2 Cell Culture Lab, 2nd floor===
Line 62: Line 78:
#'''Liquid Nitrogen'''
#'''Liquid Nitrogen'''


===The Dog House, Bindley Room 152===
 
#'''Nitric Oxide'''  Nitric Oxide (NO) is a Class A dangerous gas which could be inhaled without any obvious external indications (i.e. you might not know it).  Nitric oxide can be directly toxic to blood, lungs, nervous system, pancreas and skin.  NEVER intentionally inhale NO.  NO should not be confused with the "laughing gas", nitrous oxide N2O, that you get at the dentist!
#'''Laboratory Scale'''  Please be sure to clean up the scale after use.  This includes washing the weighing utensils, brushing off any spilled chemicals, and using a wet paper towel, if necessary.
#'''pH Meter'''  Please read and understand the instructions prior to use. If you are unsure of how to PROPERLY use the meter, please ask for assistance.  We have recently had to purchase a new pH probe due to misuse.


===The Dungeon, Bindley Room B025===
===The Dungeon, Bindley Room B025===
Line 72: Line 85:
#'''Gas Cylinders'''  Gad cylinders can serve as projectiles if damaged or knocked over.  All cylinders must be properly secured using a chain or a strap.  In addition, all cylinders must be either capped during storage or have a regulator during use.  There should not be any uncapped cylinders in the lab or storage areas.  If you need to handle gas cylinders, you must go through [http://www.purdue.edu/rem/safety/saftrain.htm#cg REM training].
#'''Gas Cylinders'''  Gad cylinders can serve as projectiles if damaged or knocked over.  All cylinders must be properly secured using a chain or a strap.  In addition, all cylinders must be either capped during storage or have a regulator during use.  There should not be any uncapped cylinders in the lab or storage areas.  If you need to handle gas cylinders, you must go through [http://www.purdue.edu/rem/safety/saftrain.htm#cg REM training].
#'''Biological Organisms.'''  A range of biological samples including bacteria, biofilms, and mammalian cells lines are used (but not cultured) in B025.  If you are working in this area you should read the biological safety handbook and heed all signage and biohazard labels.  This reality also means that cross contamination between cells samples is possible.  B025 should be regarded as a "terminal experiment" location.  Cell cultures should generally not be returned to culture.
#'''Biological Organisms.'''  A range of biological samples including bacteria, biofilms, and mammalian cells lines are used (but not cultured) in B025.  If you are working in this area you should read the biological safety handbook and heed all signage and biohazard labels.  This reality also means that cross contamination between cells samples is possible.  B025 should be regarded as a "terminal experiment" location.  Cell cultures should generally not be returned to culture.
#'''Nitric Oxide'''  Nitric Oxide (NO) is a Class A dangerous gas which could be inhaled without any obvious external indications (i.e. you might not know it).  Nitric oxide can be directly toxic to blood, lungs, nervous system, pancreas and skin.  NEVER intentionally inhale NO.  NO should not be confused with the "laughing gas", nitrous oxide N2O, that you get at the dentist!
#'''Laboratory Scale'''  Please be sure to clean up the scale after use.  This includes washing the weighing utensils, brushing off any spilled chemicals, and using a wet paper towel, if necessary.
#'''pH Meter'''  Please read and understand the instructions prior to use. If you are unsure of how to PROPERLY use the meter, please ask for assistance.  We have recently had to purchase a new pH probe due to misuse.


==Safety Training Log==
==Safety Training Documentation==
 
===Current Students===
*Tom Moffitt (joined September 2007)
#Read chemical hygiene plan
 
*Subhashree Mohanty, MS student (joined Jan 2007)
 
*Carmen (Shuai) Zhang, MS student
 
*Craig Barcus, BS / MS student
# Read Chemical Hygiene Plan (May 2008)
# Read Purdue Biological Safety Manual (May 2008)
# Read Purdue Online Biological Safety Training (May 2008)
# Read Purdue Handling Human Cell Lines (May 2008)
# Read Bindley Bioscience Center Policies (May 2008)
 
*Alexander DiMauro, MS student (joined June 2007)
#read chemical hygiene plan (June 2006, June 2007)
#REM chem safety training (June 2006)
#REM laser safety training (July 2006)
#REM radioactive materials safety training (July 2006)
#read biological safety manual (June 2007)
#REM bloodborne pathogens training (6/15/07)
 
===Former Students===
*Sue Zhao, PhD student (joined lab summer 2003)
 
*Sabrina Jedlicka, PhD student (joined lab 8/04)
#chemical hygiene
#Summer 2006, attended BME Safety Training
 
*John Koehler, MS student (joined lab 5/05
#6/05 read chemical hygiene plan
#6/05 PPE training
 
*Meghan Floyd (joined lab 10/04)
#6/06 read chemical hygiene plan, read biosafety awareness presentation
#Fall 2006, attended FS annual safety training
#Summer 2006, attended SURF safety training


*Christina Matson, BME REU student (joined 6/05, left 8/05)
IMPORTANT.  Please turn in any and all documentation regarding safety training to Dr. Rickus.
#6/9/05 REU, chemical hygiene training, read plan
#6/9/05 REU, PPE training

Latest revision as of 15:42, 15 September 2015

Click here to return to main Rickus Lab page

EMERGENCY CONTACTS

In a real emergency, call 911

Case Specific Purdue Emergency Contacts

Prof. Rickus 494-1197

Important Safety Information Links

Purdue Biological Safety Manual

On-line Biological Safety Training

Handling Human Cell Lines

Purdue Chemical Hygiene Plan

Bindley Bioscience Center Building Policies

Purdue REM Laser Safety

Birck Nanotechnology Training

CDC Biosafety Publication

Safety Requirements for All Lab Members

All new lab members must:

  1. Go through the Bindley Bioscience Center new user process (form to be completed, meet with building staff)
  2. Read the Purdue Chemical Hygiene Plan
  3. Sign the Chemical Hygiene Awareness Certificate. Turn in to Dr. Rickus
  4. Read the Purdue Biological Safety Manual
  5. Familiarize themselves with the Specific Safety Notes below for the areas in which they will be working
  6. Take note of the Hazard Assessment Sheet on the door of your work area
  7. Locate the MSDS books in your work area
  8. Check in with Dr. Rickus to discuss and document the completion of these steps.

If you will be working in Birck, you must go through Birck Specific Training in Addition to the steps above"'

  1. Click here to sign up for Birck Training

If you will be handling or working around biological samples. You must

  1. Complete the on-line Biological Safety Training
  2. Familiarize yourself with the Bindley Bioscience Center Policies
  3. Discuss any particular hazards and the safety level of your work with Dr. Rickus
  4. Complete and sign the biological safety training certificate. Turn in the form to Dr. Rickus.

If you will be working in BSL2 areas of Bindley or Birck, you must

  1. Complete bloodborne pathogen training. You can complete this trough REM. Click here for info. or through Lisa Reese in Birck. Click Here to register

If you will be working with or around human cell lines (e.g. Caco-2, GAMB1) you must also:

  1. Read the Handling Human Cell Lines presentation Handling Human Cell Lines
  2. Complete and sign the human cell line certification form located at the end of the training presentation.
  3. Note that all human cell lines are considered at least BSL2, so you must complete bloodborne pathogen training.

Special Safety Notes of Particular Relevance to the Rickus Lab

general lab handling

  1. Birck Nanotechnology Center. Absolutely NO biological materials are to enter Birck without explicit permission. To obtain permission and policies, please see Lisa Reece in the Molecular Cytometry Labs. lreece@purdue.edu
  2. Dry Ice. Many of our incoming shipments will arrive packed in dry ice. Dry ice is solid carbon dioxide. Dry ice is extremely cold. Special care should be taken. Use insulated gloves to handle the dry ice. In addition, dry ice required good ventilation in the room. The solid CO2 will sublimate into gaseous CO2 which can fill a closed space and result in suffication.

Biomolecules Lab, Bindley Room 234

  1. piranha solution. This solution is commonly used in the biopolymer chemical area to clean residual organics off of glass slides. It consists of sulfuric acid and H2O2 at a 3:1 ratio. It is a strong oxidizer. Do not handle pirahna unless you have been properly trained by Dr. Rickus. Some special safety notes regarding pirhana: Always prepare in the hood, with proper gloves, goggles, and lab coat. Piranha can cause severe burns. Never mix with organic solvents. Reactions are extremely exothermic. Piranha should not be stored. Make solutions fresh. Never seal in a closed container; explosions are possible. It should be used for removing only residual and trace levels of organic contamination. There are forceps and beakers that are set aside for use of piranha solution. Please do not use these materials for any other use!!!

Links to other university safety information regarding piranha. Princeton EHS U. of Maryland Tufts

Molecular Biology, Bindley 134

  1. ethidium bromide. Ethidium bromide (EtBr), 3,8-diamino-5-ethyl-6-phenylphenanthridinium bromide, is commonly used as a non-radioactive marker for identifying and visualizing nucleic acid bands in electrophoresis and other methods of gel-based nucleic acid separation. EtBr is a dark red crystalline that readily fluoresces reddish-brown when exposed to ultraviolet (UV) light. EtBr is a potent mutagen and toxic after an acute exposure. It is an irritant to the skin, eyes, mouth, and upper respiratory tract. Direct contact with EtBr should be avoided. Read REM's safety information for proper handling and disposal of ethidium bromide

BSL2 Cell Culture Lab, 2nd floor

  1. Training NO ONE is to enter this room without training and permission.
  2. Cell Lines. All cells in 158 are Biosafety Level 1 or 2 cells. BSL2 procedures should be use at all times for all work in this lab.
  3. Gas Cylinders Gad cylinders can serve as projectiles if damaged or knocked over. All cylinders must be properly secured using a chain or a strap. In addition, all cylinders must be either capped during storage or have a regulator during use. There should not be any uncapped cylinders in the lab or storage areas. If you need to handle gas cylinders, you must go through REM training.
  4. Liquid Nitrogen


The Dungeon, Bindley Room B025

  1. Class IIIb lasers. Lasers are used in B025. You must head all posted signage regarding lasers. You must be trained and certified to use the lasers. No untrained personnel should touch an laser equipment. Please read the laser safety information provided by REM. Purdue Laser Safety
  2. Gas Cylinders Gad cylinders can serve as projectiles if damaged or knocked over. All cylinders must be properly secured using a chain or a strap. In addition, all cylinders must be either capped during storage or have a regulator during use. There should not be any uncapped cylinders in the lab or storage areas. If you need to handle gas cylinders, you must go through REM training.
  3. Biological Organisms. A range of biological samples including bacteria, biofilms, and mammalian cells lines are used (but not cultured) in B025. If you are working in this area you should read the biological safety handbook and heed all signage and biohazard labels. This reality also means that cross contamination between cells samples is possible. B025 should be regarded as a "terminal experiment" location. Cell cultures should generally not be returned to culture.
  4. Nitric Oxide Nitric Oxide (NO) is a Class A dangerous gas which could be inhaled without any obvious external indications (i.e. you might not know it). Nitric oxide can be directly toxic to blood, lungs, nervous system, pancreas and skin. NEVER intentionally inhale NO. NO should not be confused with the "laughing gas", nitrous oxide N2O, that you get at the dentist!
  5. Laboratory Scale Please be sure to clean up the scale after use. This includes washing the weighing utensils, brushing off any spilled chemicals, and using a wet paper towel, if necessary.
  6. pH Meter Please read and understand the instructions prior to use. If you are unsure of how to PROPERLY use the meter, please ask for assistance. We have recently had to purchase a new pH probe due to misuse.

Safety Training Documentation

IMPORTANT. Please turn in any and all documentation regarding safety training to Dr. Rickus.

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