Rickus Lab Safety

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Important Safety Information Links

Purdue Biological Safety Manual

On-line Biological Safety Training

Handling Human Cell Lines

Purdue Chemical Hygiene Plan

Special Safety Notes of Particular Relevance to the Rickus Lab

general lab handling

1. Dry Ice. Many of our incoming shipments will arrive packed in dry ice. Dry ice is solid dioxide. Dry ice is extremely cold. Special care should be taken. Use insulated gloves to handle the dry ice. In addition, dry ice required good ventilation in the room. The solid CO2 will sublimate into gaseous CO2 which can fill a closed space and result in suffication.

Biopolymers Lab, Bindley Room 234

1. pyrahna solution.

Cell Culture Lab, Bindley Room 158

1. Cell Lines. Currently all cells in 158 are Biosafety Level 1 (BSL1) cells. Please note that waste generated from BSL1 culture is treated as "look-a-like" waste, but it not a biohazard. Please do not use red autoclave bags or biohazard labels on BSL1 waste.
2. Gas Cylinders Gad cylinders can serve as projectiles if damaged or knocked over. All cylinders must be properly secured using a chain or a strap. In addition, all cylinders must be either capped during storage or have a regulator during use. There should not be any uncapped cylinders in the lab or storage areas.

Safety Training Log

Current Students

• Sue Zhao, PhD student (joined lab summer 2003)

• Sabrina Jedlicka, PhD student (joined lab 8/04)

1. chemical hygiene

• John Koehler, MS student (joined lab 5/05

1. 6/05 read chemical hygiene plan
2. 6/05 PPE training

• Meghan Floyd (joined lab 10/04)

1. 6/06 read chemical hygiene plan, read biosafety awareness presentation
2. Fall 2006, attended FS annual safety training
3. Summer 2006, attended SURF safety training

Former Students

• Christina Matson, BME REU student (joined 6/05, left 8/05)

1. 6/9/05 REU, chemical hygiene training, read plan
2. 6/9/05 REU, PPE training