Rifampicin: Difference between revisions
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==Mode of Action== | ==Mode of Action== | ||
Strongly inhibits prokaryotic RNA polymerase and mammalian RNA polymerase to a lesser degree. | Strongly inhibits prokaryotic RNA polymerase and mammalian RNA polymerase to a lesser degree. Does not inhibit T7 RNA polymerase. | ||
==Mechanism of Resistance== | ==Mechanism of Resistance== | ||
Revision as of 22:01, 17 December 2005
Mode of Action
Strongly inhibits prokaryotic RNA polymerase and mammalian RNA polymerase to a lesser degree. Does not inhibit T7 RNA polymerase.
Mechanism of Resistance
Cells can spontaneously mutate to achieve resistance to Rifampicin.
Usage Notes
- 50 mg/mL stock solution in methanol.
- Vortex immediately to prevent rifampicin from sticking to bottom of tube.
- Add ~5 drops 10 N NaOH per mL to facilitate dissolving.
- Add 2 mL per liter of culture to achieve a working concentration of 100 μg/mL.
Rifampicin is light-sensitive so store stock solution, plates and media in the dark and wrap in foil.
