Rifampicin - Revision history

Reshma P. Shetty at 17:48, 26 July 2007

Reshma P. Shetty — Thu, 26 Jul 2007 17:48:08 GMT

←Older revision		Revision as of 17:48, 26 July 2007
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	==References==		==References==
-
	*[[A Short Course in Bacterial Genetics]]		*[[A Short Course in Bacterial Genetics]]
	*[[Molecular Cloning]]		*[[Molecular Cloning]]
		+
		+	[[Category:Material]] [[Category:Antibiotic]]

Madhadron at 23:16, 17 March 2007

Madhadron — Sat, 17 Mar 2007 23:16:45 GMT

←Older revision		Revision as of 23:16, 17 March 2007
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	#Add 2 mL per liter of culture to achieve a working concentration of 100 μg/mL.		#Add 2 mL per liter of culture to achieve a working concentration of 100 μg/mL.

-	Rifampicin is light-sensitive so store stock solution, plates and media in the dark and wrap in foil.	+	Alternately, dissolve 50mg/mL in DMSO and vortex to suspend. In this form, you don't need NaOH. Store at -20°C.
		+
		+	Rifampicin is light-sensitive so store stock solution, plates and media in the dark and wrap in foil. It also degrades in solution fairly quickly, so make stocks and replace them every couple months.

	==References==		==References==

Kathmc: /* Mode of Action */

Kathmc — Sun, 18 Dec 2005 05:01:31 GMT

Mode of Action

←Older revision		Revision as of 05:01, 18 December 2005
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	==Mode of Action==		==Mode of Action==

-	Strongly inhibits prokaryotic RNA polymerase and mammalian RNA polymerase to a lesser degree.	+	Strongly inhibits prokaryotic RNA polymerase and mammalian RNA polymerase to a lesser degree. Does not inhibit T7 RNA polymerase.

	==Mechanism of Resistance==		==Mechanism of Resistance==

Barry Canton: Suggestion for how to handle common references. Change back if desired.

Barry Canton — Mon, 23 May 2005 20:58:02 GMT

Suggestion for how to handle common references. Change back if desired.

←Older revision		Revision as of 20:58, 23 May 2005
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	==References==		==References==

-	~~J. H. Miller. ''~~A Short Course in Bacterial Genetics~~: A Laboratory Manual and Handbook for Escherichia coli and Related Bacteria.'' Cold Spring Harbor Laboratory Press, 1992.~~	+	*[[A Short Course in Bacterial Genetics]]
-		+	*[[Molecular Cloning]]
-	~~J. Sambrook and D. W. Russell. ''~~Molecular Cloning~~: A Laboratory Manual.'' Cold Spring Harbor Laboratory Press, 3rd edition, 2001.~~	+

Reshma P. Shetty at 20:34, 23 May 2005

Reshma P. Shetty — Mon, 23 May 2005 20:34:42 GMT

New page

==Mode of Action==

Strongly inhibits prokaryotic RNA polymerase and mammalian RNA polymerase to a lesser degree.

==Mechanism of Resistance==

Cells can spontaneously mutate to achieve resistance to Rifampicin.

==Usage Notes==

#50 mg/mL stock solution in methanol.
#Vortex immediately to prevent rifampicin from sticking to bottom of tube.
#Add ~5 drops 10 N NaOH per mL to facilitate dissolving.
#Add 2 mL per liter of culture to achieve a working concentration of 100 μg/mL.

Rifampicin is light-sensitive so store stock solution, plates and media in the dark and wrap in foil.

==References==

J. H. Miller. ''A Short Course in Bacterial Genetics: A Laboratory Manual and Handbook for Escherichia coli and Related Bacteria.'' Cold Spring Harbor Laboratory Press, 1992.

J. Sambrook and D. W. Russell. ''Molecular Cloning: A Laboratory Manual.'' Cold Spring Harbor Laboratory Press, 3rd edition, 2001.