RobWardenWNB:M3D6

Expression Engineering, Day 6, 4/25/07

Microarray Analysis

Red Signal

NY389

Green Signal

FY2068

Notes

1. Copied only GeneName, SystematicName, Description, gMeanSignal, rMeanSignal gMedianSignal, rMedianSignal, gBGMeanSignal, rBGMeanSignal, gBGMedianSignal, rBGMedianSignal onto Analysis worksheet
2. Deleted Unused Controls
   • NegativeControls
   • EQCs
   • A_##_P##
3. Corrected for Background
   • BGCor = MedianSignal - BGMedianSignal
   • Gave LOTS of netative numbers for green
4. Normalized Data
   • rNorm = rBGCor * avg(gBGCor) / avg(rBGCor)