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| ==20.109 Spring 2007== | | ==Tsourkas Lab Notebooks== |
| | *[[User:Robwarden/Notebook/Caspase-3 Activatable Probe|Caspase-3 Activatable Probe]] |
| | *[[User:Robwarden/Notebook/Side Projects & Assistance|Side Projects & Assistance]] |
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| ===Last Name=== | | ==Griffith Lab Notebooks== |
| Warden
| | *[[User:Robwarden/Notebook/Divalent Ligand Validation|Divalent Ligand Validation]] |
| | *[[User:Robwarden/Notebook/GFP-RGD Force Sensor|GFP-RGD Force Sensor]] |
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| ===First Name=== | | ==20.109 Spring 2007== |
| Robert
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| ===Preferred name===
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| Rob
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| ===Course/Minor===
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| 20/5
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| ===Year of Graduation===
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| 2009
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| ===Telephone #===
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| (248) 802-7023
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| ===Email===
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| rw27 AT mit DOT edu
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| ===I have taken===
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| 5.07 (Biochemistry I)<br>
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| 7.03 (Genetics)<br>
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| ===I am taking===
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| 20.109 (Lab Fundamentals of Biological Engineering)<br> | |
| 5.03 (Inorganic Chemistry)<br>
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| 5.08 (Biochemistry II)<br>
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| 6.00 (Intro to Computer Science)<br>
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| 14.01 (Microeconomics)<br>
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| Do you have any experience culturing cells (mammalian, yeast or microbial)?<br>
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| Yes, LNCaP and HMEC human cancer cells
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| Do you have any experience in molecular biology (electrophoresis, PCR, etc)?<br>
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| Yes, electrophoresis, westerns, PCR, restriction digests, ligations, transformations, transfections
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| ===Please briefly describe any previous laboratory experience===
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| Summer UROP creating a vector with a tagged version of the growth receptor Her2, to study crosstalk in prostate cancer.
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| ==="Spit on a bat before using it for the 1st time to make it lucky."===
| | [[/Notes from 20.109|Notes from 20.109]] |
| I would give a player 5 bats that have been spit on and 5 that have not. He would then rotate through the bats, not knowing which were lucky. I would then count the number of home runs hit with each bat, and see if the spit on bats had more home runs attributed to them.
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| ==Lab Notes== | | ==Cloning Checklist== |
| ===Wiki Notebook===
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| *Expression Engineering
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| **[[RobWardenWNB:M3D1|Day 1]]
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| ***Choosing a Saga Gene
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| ***Primer Design
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| ***PCR
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| **[[RobWardenWNB:M3D2|Day 2]]
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| ***Counting Yeast
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| ***Plating Yeast
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| ***Competent Cells
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| ***Transformation
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| **[[RobWardenWNB:M3D3|Day 3]]
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| **[[RobWardenWNB:M3D4|Day 4]]
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| **[[RobWardenWNB:M3D5|Day 5]]
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| **[[RobWardenWNB:M3D6|Day 6]]
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| *Biomaterials Engineering
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| **[[RobWardenWNB:M4D1|Day 1]]
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| **[[RobWardenWNB:M4D2|Day 2]]
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| ===For Next Time===
| | [[Cloning Checklist]] |
| *Genome Engineering
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| **[[RobWarden:M13Redo|M13 Redo]]
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| **[[RobWarden:Lab1|Day 1]]
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| **[[RobWarden:M1D2|Day 2]]
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| **[[RobWarden:M1D3|Day 3]]
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| **[[RobWarden:M1D4|Day 4]]
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| *Biophysical Signal Measurement
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| **[[RobWarden:M2D1|Day 1]]
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| **[[RobWarden:M2D2|Day 2]]
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| *Expression Engineering
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| **[[RobWarden:M3Assignment|Writing Assignment]]
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| **[[RobWarden:M3D1|Day 1]]
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| *Biomaterials Engineering
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| **[[RobWarden:M4D1|Day 1]]
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