Robert W Arnold Week 12: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
 
(8 intermediate revisions by 2 users not shown)
Line 3: Line 3:


[[BIOL368/F11:Week 12 | Week 12 Assignment]]
[[BIOL368/F11:Week 12 | Week 12 Assignment]]
===Master SpreadSheet===
[[Media:Alex_Robert_Zeb_MasterSpreadSheet_20111130_CA.xls|Master Spread Sheet]]


===Journal Club===
===Journal Club===
Line 8: Line 12:
*Brought up microarray data from MRSA paper.
*Brought up microarray data from MRSA paper.
*Downloaded microarray data.
*Downloaded microarray data.
===Uploaded Data===
====Experiment E-BUG Data====
*[[Media:E-BUGS-65.README112.txt |  ReadMe]]
*[[Media:E-BUGS-65.processed.1.zip | Processed]]
*[[Media:E-BUGS-65.raw.1.zip | Raw]]
*[[Media:E-BUGS-65.sdrf.xls | SRDF]]
====Array Data====
*[[Media:A-BUGS-17.README.txt | ReadMe]]
*[[Media:A-BUGS-17.adf.xls | ADF]]
===Week 12 Assignment===
#What samples did they collect and use for the microarray experiment?
#*3 replicates of control culture and ranalexin were used in the microarray experiment.  6 total arrays were used in analysis.
#How many microarray chips did they hybridize in the experiment?
#*2 microarray chips were used.
#Which samples were paired to hybridize on the chip?
#*Ranalexin (A1) and MRSA-252(A2). MRSA-252(A1) and Ranalexin(A2).
#Which was labeled red (Cy5)?  Which was labeled green (Cy3)?
#*Ranalexin (A1) and MRSA-252(A2) was labeled red (Cy5).  Ranalexin (A2) and MRSA-252(A1) was labeled green (Cy3).
#How many replicates did they perform of each type?
#*2 technical replicates of each type.
*Arrary data file names
**Ranalexin (A1) - MBAD_12054.txt
**Ranalexin (A2) - MBAD_12061.txt
**MRSA-252 (A1) - MBAD_12055.txt
**MRSA-252 (A2) - MBAD_12060.txt
===Spreadsheet===
*Spreadsheet can be found [[Media:MasterSpreadsheet.xls | here]].


==Links==
==Links==

Latest revision as of 11:07, 30 November 2011

Electronic Lab Notebook 12

Robert W Arnold

Week 12 Assignment

Master SpreadSheet

Master Spread Sheet

Journal Club

  • Finished up journal club.
  • Brought up microarray data from MRSA paper.
  • Downloaded microarray data.

Uploaded Data

Experiment E-BUG Data

Array Data


Week 12 Assignment

  1. What samples did they collect and use for the microarray experiment?
    • 3 replicates of control culture and ranalexin were used in the microarray experiment. 6 total arrays were used in analysis.
  2. How many microarray chips did they hybridize in the experiment?
    • 2 microarray chips were used.
  3. Which samples were paired to hybridize on the chip?
    • Ranalexin (A1) and MRSA-252(A2). MRSA-252(A1) and Ranalexin(A2).
  4. Which was labeled red (Cy5)? Which was labeled green (Cy3)?
    • Ranalexin (A1) and MRSA-252(A2) was labeled red (Cy5). Ranalexin (A2) and MRSA-252(A1) was labeled green (Cy3).
  5. How many replicates did they perform of each type?
    • 2 technical replicates of each type.
  • Arrary data file names
    • Ranalexin (A1) - MBAD_12054.txt
    • Ranalexin (A2) - MBAD_12061.txt
    • MRSA-252 (A1) - MBAD_12055.txt
    • MRSA-252 (A2) - MBAD_12060.txt

Spreadsheet

  • Spreadsheet can be found here.

Links

Biol 368 Homepage

Retrieved from "https://openwetware.org/mediawiki/index.php?title=Robert_W_Arnold_Week_12&oldid=569226"