Ryan N. Willhite Week 12: Difference between revisions
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**=(B4-$B$2)/$B$3 | **=(B4-$B$2)/$B$3 | ||
===Perform Statistical Analysis on the ratios=== | |||
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TDIST(ABSCR2), (degrees of freedom, 2) | TDIST(ABSCR2), (degrees of freedom, 2) | ||
** degree of freedom= #of replicates-1 | ** degree of freedom= #of replicates-1 | ||
===Sanity Check: Number of genes significantly changed=== | |||
[[Image:Rnwwork413.xls]] | [[Image:Rnwwork413.xls]] | ||
Revision as of 15:50, 19 April 2010
Work day
Normalize Ratios
- Add rows
- Type Equations
- =AVERAGE( : )
- =STDEV( : )
- Insert columns and input equation
- (cell 4-average)/Stdev)
- Scaling and centering
- =(B4-$B$2)/$B$3
Perform Statistical Analysis on the ratios
- Average log fold change for replicates
- =AVERAGE(B2:E2)
- Make columns "Avg_LogFC_all"
- New column "Tstat"- T statistic
=AVERAGE (N2:P2)/ STDEV (N2:P2)/SQRT (#of replicates)
- New column "Pvalue."
TDIST(ABSCR2), (degrees of freedom, 2)
- degree of freedom= #of replicates-1
