Ryan N. Willhite Week 12

• [[Media:Saureus_import_all.txt | S. aureus data ready for import into GenMAPP

Work day

Normalize Ratios

1. Add rows
2. Type Equations

• =AVERAGE( : )
• =STDEV( : )

1. Insert columns and input equation

• (cell 4-average)/Stdev)
• Scaling and centering
  • =(B4-$B$2)/$B$3

Perform Statistical Analysis on the ratios

• Average log fold change for replicates
  • =AVERAGE(B2:E2)

• Make columns "Avg_LogFC_all"

• New column "Tstat"- T statistic

=AVERAGE (N2:P2)/ STDEV (N2:P2)/SQRT (#of replicates)

• New column "Pvalue."

TDIST(ABSCR2), (degrees of freedom, 2)

• • degree of freedom= #of replicates-1

Sanity Check: Number of genes significantly changed

File:Rnwwork413.xls